We narrowed to 10,796 results for: HA

1. mTFP1-Farnesyl-5

   Plasmid

   #55481

   Purpose

   Localization: Plasma Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. nAChR beta2 CFP

   Plasmid

   #15106

   Depositor

   Henry Lester

   Article

   Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

   Insert

   beta2-CFP-M (Chrnb2 Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pUC19-E6MBW6

   Plasmid

   #103102

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

   Plasmid

   #74606

   Purpose

   expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Y101A, Y103A, W120A

   Promoter

   SFFV

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pF713-pEGFP-N1-Hs-FL-METTL20 wt

   Plasmid

   #85114

   Purpose

   transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-7 asyn S129G

   Plasmid

   #36049

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein S129G (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S129G

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFRT-DHX36iso2E335A

   Plasmid

   #159588

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLCHKO_GFP_Luciferase

   Plasmid

   #155079

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80060

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mPapaya1-Farnesyl-5

    Plasmid

    #56650

    Purpose

    Localization: Membrane, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-CHRFAM7ADelta2bp

    Plasmid

    #62515

    Purpose

    mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)

    Depositor

    Sherry Leonard, Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7ADelta2bp (CHRFAM7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partially duplication of CHRNA7 with 2-bp deletio…

    Promoter

    CMV

    Available since

    March 20, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. PABPN1L

    Plasmid

    #155807

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PABPN1L (PABPN1L Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

    Plasmid

    #205444

    Purpose

    human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-HLA-E SCT-hCD55
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. nAChR alpha4 CFP

    Plasmid

    #15244

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    alpha4-CFP-M (Chrna4 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-hSyn-mSyp-RT1-24xGCN4

    Plasmid

    #106300

    Purpose

    The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    mSyp-RT1-24xGCN4 (Syp Mouse)
    

    Use

    Tags

    24xGCN4

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF708-pET28a-Hs-delta38-METTL20-NHis wt

    Plasmid

    #85109

    Purpose

    expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta38) METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge