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pHBDuet140

Plasmid

#121914

Purpose

Orthogonal NpuDnaB mini-intein intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV RhoB YFP MS2

Plasmid

#131777

Purpose

RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

CRISPR

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV Btg2 YFP MS2

Plasmid

#131776

Purpose

Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

CRISPR

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-BRAT-EnR

Plasmid

#117252

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1997 May;124(9):1689-98.

Insert

BRAT-EnR (vegt-a Frog)

Use

Xenopus expression

Mutation

chimeric protein with engrailed repressor domain…

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

Plasmid

#127409

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127399

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-dCas9_O

Plasmid

#127028

Purpose

Basic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part dCas9_O

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0033-MV_RN

Plasmid

#127016

Purpose

Basic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0033m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-RBS-pET-MV_RN

Plasmid

#127014

Purpose

Basic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part RBS-pET-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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JK104: pMVP-DEST

Plasmid

#121851

Purpose

pMVP destination vector, empty expression plasmid backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH401: pMVP (L5-L4) CMV promoter

Plasmid

#121726

Purpose

pMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HE302: pMVP (L5-L4) RIP

Plasmid

#121728

Purpose

pMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

RIP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO502: pMVP (R4-R3) eCFP

Plasmid

#121734

Purpose

pMVP R4-R3 entry plasmid, contains eCFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO301: pMVP (R4-R3) eYFP

Plasmid

#121736

Purpose

pMVP R4-R3 entry plasmid, contains eYFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HD301: pMVP (R4-R3) βgal

Plasmid

#121738

Purpose

pMVP R4-R3 entry plasmid, contains βgal for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

βgal

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HM001: pMVP (R4-R3) Cre

Plasmid

#121740

Purpose

pMVP R4-R3 entry plasmid, contains Cre for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Cre

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Tac

Plasmid

#126958

Purpose

Terminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

Plasmid

#127408

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

Plasmid

#127406

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, K305H,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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