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pEBB-3XMyc-TRAF2 S11A

Plasmid

#44105

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 11 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pDEST-actc1b-ccdb-mKate2

Plasmid

#109731

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-mKate2-ccdb

Plasmid

#109732

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer2

Plasmid

#14777

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pBGSA FPR1

Plasmid

#62600

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (FPR1 Human)

Expression

Mammalian

Promoter

SRalpha

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only
pLib6.4B

Plasmid

#176665

Purpose

Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TRmiRc2

Plasmid

#25750

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts[mCherry]-1A(lacZ)

Plasmid

#126980

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJM111

Plasmid

#134162

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 295-C (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet P2A Rac1-wt

Plasmid

#125135

Purpose

Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mNeon/mScarletP2A-Rac1

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(26/19) AB(monomer), wt

Plasmid

#22381

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pYFP (GB0053)

Plasmid

#68197

Purpose

Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A) C716A

Plasmid

#24605

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, C716A

Available Since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pMC-6-RLuc-9

Plasmid

#197739

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Renilla luciferase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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actb2-lifeact-mKate2

Plasmid

#109750

Purpose

Ubiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the actin binding lifeact sequence

Use

Zebrafish plasmids

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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XE152 GFP-XDpr-CS2+

Plasmid

#16770

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

GFP-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pJUMP18-pheS_NOC

Plasmid

#127031

Purpose

Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part PheS_NOC

Use

Synthetic Biology

Expression

Bacterial

Mutation

pheS A294G promiscuous mutation

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192396

Purpose

To act as a 2-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192391

Purpose

To act as a 2-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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