We narrowed to 5,913 results for: pcDNA 3.1

1. Collybistin scFv [L120/12]

   Plasmid

   #190507

   Purpose

   Mammalian Expression of Collybistin scFV. Derived from hybridoma L120/12.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Collybistin (Rattus norvegicus) recombinant scFV (Arhgef9 Mouse)
   

   Use

   Tags

   Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Kv1.3 K+ channel scFv [L23/27]

   Plasmid

   #190514

   Purpose

   Mammalian Expression of Kv1.3 K+ channel scFV. Derived from hybridoma L23/27.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv1.3 K+ channel (Rattus norvegicus) recombinant scFV (Kcna3 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Kv2.1 K+ channel scFv [K89/34]

   Plasmid

   #190494

   Purpose

   Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma K89/34.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv2.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RGS14 scFv [N133/21]

   Plasmid

   #190529

   Purpose

   Mammalian Expression of RGS14 scFV. Derived from hybridoma N133/21.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   RGS14 (Rattus norvegicus) recombinant scFV (Rgs14 Mouse)
   

   Use

   Tags

   Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Kv1.1 K+ channel scFv [K20/78]

   Plasmid

   #190486

   Purpose

   Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GABA(A)R, Gamma2L/S scFv [N452/73]

   Plasmid

   #190555

   Purpose

   Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GABA(A)R, Gamma2L/S scFv [N452/81]

   Plasmid

   #190556

   Purpose

   Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/81.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

   Plasmid

   #185701

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(FCA)
   

   Use

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

   Plasmid

   #185705

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(PDCA)
   

   Use

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

    Plasmid

    #185703

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(SCA)
    

    Use

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

    Plasmid

    #185704

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PCA)
    

    Use

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

    Plasmid

    #185702

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(BCA)
    

    Use

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Arl13b scFv [N295B/66]

    Plasmid

    #182086

    Purpose

    Mammalian Expression of Arl13b scFv. Derived from hybridoma N295B/66.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Arl13b (Mus musculus) (Arl13b Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RGS14 scFv [N133/21]

    Plasmid

    #182081

    Purpose

    Mammalian Expression of RGS14 scFv. Derived from hybridoma N133/21.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting RGS14 (Rattus norvegicus) (Rgs14 Mouse)
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Kv1.2 K+ channel scFv [K14/16]

    Plasmid

    #182064

    Purpose

    Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CXCR4-SZ158a-sfGFP

    Plasmid

    #162446

    Purpose

    Expression in HEK293T cell and compete ligand singaling against full-length receptors

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-X-C chemokine receptor type 4 (CXCR4 Human)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    truncation from aa52 to aa257

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid

    #103871

    Purpose

    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lucMAPT-GenRep

    Plasmid

    #214674

    Purpose

    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. peGFP NOVA2 op

    Plasmid

    #224354

    Purpose

    Express GFP-tagged human NOVA2 (codon optimized)

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

    Insert

    NOVA2 (NOVA2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    codon optimized for expression in human cells

    Promoter

    CMV and T7

    Available since

    Sept. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge