We narrowed to 8,333 results for: Set;

1. ptRNAfMet-A37

   Plasmid

   #196657

   Purpose

   Plasmid for overexpression of native initiator tRNA (A37)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37 expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   metY native promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-mGreenLantern

   Plasmid

   #219643

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKAd5f11pES-PmeI

   Plasmid

   #122698

   Purpose

   To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

   Depositor

   Zhuo-Zhuang Lu

   Article

   Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB8786

   Plasmid

   #126920

   Purpose

   Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. OCT4-2A-mOrange

   Plasmid

   #66986

   Purpose

   Generate OCT4 mOrange reporter line without selection cassette

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   2A-mOrange
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2SK

   Plasmid

   #59383

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-kan
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

   Plasmid

   #179027

   Purpose

   Parent vector for markerless yeast integration cassettes with untagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-FlEx switch

    Plasmid

    #82406

    Purpose

    Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV-40

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGD248

    Plasmid

    #219498

    Purpose

    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSkipFlox

    Plasmid

    #85797

    Purpose

    P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    split Cre recombinase
    

    Use

    Cre/Lox; P. falciparum expression

    Tags

    FKBP, FRB*

    Expression

    Mutation

    Promoter

    Pfcrt

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHMattR3-attR4-GFP

    Plasmid

    #196247

    Purpose

    pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgDonor_lentiGuide

    Plasmid

    #184168

    Purpose

    LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Insert

    sgDonor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAbaMCi-GFP-gfp_pJMP2768

    Plasmid

    #208889

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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