Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 23,735 results for: fus

Clear keywords
Showing: 8821 - 8850 of 23735 results

  1. pMP71-mGFP-llo190-mc38minor

    Plasmid
    #174604
    Purpose
    expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1
    Depositor
    Stanley Riddell
    Article
    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441
    Insert
    palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
    Expression
    Mammalian
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    174604-plasmid-map-sequence-id-344450

    Enlarge

  2. pBbB8k-csg-NbSARS

    Plasmid
    #171703
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171703-plasmid-map-sequence-id-336471

    Enlarge

  3. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133016-plasmid-map-sequence-id-262984

    Enlarge

  4. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

    Enlarge

  17. pMOD_B3200

    Plasmid
    #91079
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    none (begins with P2A to be fused to TALE-VPR_1)
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91079-plasmid-map-sequence-id-182042

    Enlarge

  18. pMOD_B3400

    Plasmid
    #91080
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    none (begins with P2A to be fused to TALE-VP64_1)
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91080-plasmid-map-sequence-id-182043

    Enlarge

  19. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Tags
    YFP
    Expression
    Bacterial
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

    Enlarge

  20. RGD-I27-CC

    Plasmid
    #85393
    Purpose
    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85393-plasmid-map-sequence-id-172696

    Enlarge

  21. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

    Enlarge

  22. p1G108CFa

    Plasmid
    #85089
    Purpose
    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
    Promoter
    T7 promoter
    Available Since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    85089-plasmid-map-sequence-id-164483

    Enlarge

  25. pNB682

    Plasmid
    #60143
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    A4V
    Promoter
    MET17pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60143-plasmid-map-sequence-id-96214

    Enlarge

  26. pET30-GBF-E6

    Plasmid
    #21696
    Depositor
    James Baleja
    Article
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available Since
    July 28, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 8821 - 8850 of 23735 results