We narrowed to 17,707 results for: Comp;

1. pMBPCas2/3

   Plasmid

   #89238

   Purpose

   Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas2/3
   

   Use

   Tags

   Strep II-MBP

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

   Plasmid

   #79902

   Purpose

   Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Insert

   3xFLAG_Twin_Strep_EZH2
   

   Use

   CRISPR and TALEN

   Tags

   3xFLAG_Twin_Strep

   Expression

   Mammalian

   Mutation

   Promoter

   PGK1

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDFBB-eGFP

   Plasmid

   #32550

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   egfp (eGFP )
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Mutation

   Promoter

   Consitutive lac

   Available since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. JH04

   Plasmid

   #73513

   Purpose

   mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mRuby2::PH::tbb-2
   

   Use

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   mex-5

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG424 Smad2 (Gal4-Smad2)

   Plasmid

   #14932

   Depositor

   Joan Massague

   Article

   Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

   Insert

   Smad2 (SMAD2 Human)
   

   Use

   Tags

   Gal4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pC002 - LshC2C2 locus into pACYC184

   Plasmid

   #79151

   Purpose

   Endogenous LshC2c2 locus cloned into pACYC184

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. YSBE 517 : pNEO_6xHA

   Plasmid

   #92083

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selection

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   6xHA
   

   Use

   tagging proteins in cryptococcus neoforman

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. RFP-(SUMO FAAA)3

   Plasmid

   #130703

   Purpose

   Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   3 copies of human SUMO1 separated by (GGS)4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FKVK sequence in each SUMO1 module changed to FAAA

   Promoter

   CMV

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT276

   Plasmid

   #122607

   Purpose

   express CDA1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   CDA1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas1_Cas2/3

    Plasmid

    #89240

    Purpose

    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas1 and Cas2/3
    

    Use

    Tags

    Strep II tag on Cas1

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRAM1-EGFP

    Plasmid

    #210365

    Purpose

    Expresses TRAM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TRAM1 (TRAM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pscALPS gag-gfp Blasti

    Plasmid

    #115803

    Purpose

    Express gag-gfp fusion driven by SFFV promoter

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOGG010

    Plasmid

    #113984

    Purpose

    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    RK2 (IncP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Flpo

    Plasmid

    #125576

    Purpose

    Expresses Flpo under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-AsCas12f-v4.1

    Plasmid

    #204636

    Purpose

    Expression of AsCas12f v4.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f-v4.1
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D169K, N199K, N328G, D364R

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1 human TBC1D7 (19-293)

    Plasmid

    #132617

    Purpose

    expresses human TBC1D7 (19-293) in E. coli

    Depositor

    Wenqing Xu

    Article

    Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

    Insert

    TBC1D7 (19-293)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSW609 - N001

    Plasmid

    #115993

    Purpose

    intermediate vector for GreenGate cloning method, contains modules from A-F

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Unspecified

    Tags

    HDEL and SP(ER)

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEL638

    Plasmid

    #189831

    Purpose

    The T-DNA backbone of nCas12j2 for genome editing in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR; T-dna clone

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi1

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJ915v2+Nterm Spe1 +Cterm Age1 site

    Plasmid

    #88914

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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