We narrowed to 32,142 results for: eng

1. AdMKP-3 S159A

   Plasmid

   #131520

   Purpose

   Expresses MKP-3 with S159A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Mutation

   changed Serine 159 to Alanine

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. dre-cdk2 3'UTR sicheck2

   Plasmid

   #121123

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-cdk2 3'UTR (cdk2 Zebrafish)
   

   Expression

   Bacterial

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-30a(+)-C-SH2

   Plasmid

   #111419

   Purpose

   express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-30a(+)-C-SH2+IA

   Plasmid

   #111418

   Purpose

   express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-30a(+)-N-SH2+IA

   Plasmid

   #111273

   Purpose

   express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSi-check2-hRac2 3'UTR

   Plasmid

   #97160

   Purpose

   For dual luciferase report assay

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   RAC 3'UTR (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-Atoh1 del3

   Plasmid

   #96913

   Purpose

   Murine Atoh1 with amino acids 214-305 removed

   Depositor

   Albert Edge

   Article

   Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 214-305

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-Atoh1 del4

   Plasmid

   #96914

   Purpose

   Murine Atoh1 with amino acids 306-347 removed

   Depositor

   Albert Edge

   Article

   Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 306-347

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RSV/mGATA3 (antisense)

   Plasmid

   #83817

   Purpose

   contains antisense mouse GATA3

   Depositor

   Douglas Engel

   Article

   Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

   Insert

   GATA3-antisense strand (Gata3 Mouse)
   

   Expression

   Mammalian

   Promoter

   RSV

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV V5-lamin A

    Plasmid

    #175107

    Purpose

    Lentiviral expression of V5-tagged mouse Lmna

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Lmna (Lmna Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA 3.3 N-MYC RMDN3

    Plasmid

    #227878

    Purpose

    Used for transfection of cells for expression of C-MYC RMDN3

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    RMDN3 (RMDN3 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304 Flag-mCherry-eEF1A1_IRES-GFP

    Plasmid

    #198383

    Purpose

    eEF1A1 fluorescent reporter

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    eEF1A1 (EEF1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGD-p19

    Plasmid

    #196326

    Purpose

    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    tomato bushy stunt virus p19
    

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-puro-cMyc

    Plasmid

    #46970

    Purpose

    lentiviral expression of c-Myc

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lentiviral mBmal1-Luc

    Plasmid

    #182762

    Purpose

    Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

    Depositor

    Qing-Jun Meng

    Article

    Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

    Insert

    mouse arntl promoter (Bmal1 Mouse)
    

    Use

    Lentiviral

    Promoter

    649 bp

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGD-γb

    Plasmid

    #196327

    Purpose

    35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    barley stripe mosaic virus γb
    

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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