We narrowed to 40,052 results for: lat

1. pJZC638

   Plasmid

   #62283

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Nuclease activity has been deactivated by mutatio…

   Promoter

   pAdh and pGal10

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET27b-ecDHFR-N23PP/L28F

   Plasmid

   #118584

   Purpose

   Bacterial expression of E.coli DHFR N23PP and L28F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Expression

   Bacterial

   Mutation

   L28 mutated to F28, N23 mutated to P23, a proline…

   Promoter

   T7

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-alpha(740-977)-GFP

   Plasmid

   #100731

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2A1 (Ap2a1 Mouse)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_pSyn_Mito-LOV-Turbo-V5

   Plasmid

   #199669

   Purpose

   expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   AAV

   Tags

   COX4I1 mito targeting sequence and V5

   Promoter

   Synapsin

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

   Plasmid

   #199585

   Purpose

   Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
   AkaLuc
   Blasticidin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHM123DEnv

   Plasmid

   #137668

   Purpose

   Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

   Depositor

   Anjali Joshi

   Article

   Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

   Insert

   HIV-1 Envelope Glycoprotein
   

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

   Plasmid

   #89636

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   S317A, AGC-->GCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

   Plasmid

   #78352

   Purpose

   Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-KRAB
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. DHFR-GFP-th-NL1

   Plasmid

   #173007

   Purpose

   For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   DHFR-GFP-NL1
   

   Tags

   DHFR and EGFP

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6- Entry-RPL26DC-DDK

    Plasmid

    #133867

    Purpose

    Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26DC-myc-FLAG (RPL26 Human)
    

    Tags

    Myc-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHFR-GFP-th-GluA1

    Plasmid

    #173008

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-GFP-th-GluA1
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFAB3677

    Plasmid

    #47833

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 2 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-RIPK3[S227D]-FLAG

    Plasmid

    #199320

    Purpose

    mutated phosphorylation site (MLKL interaction permanent active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Aspartic Acid

    Available Since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-RIPK3[S227A]-FLAG

    Plasmid

    #199318

    Purpose

    mutated phosphorylation site (MLKL interaction inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Alanine

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT/Caggs-NRAS G12V-IRES-ASK1

    Plasmid

    #221074

    Purpose

    Expresses NRAS G12V and ASK1 in mammalian cells

    Depositor

    Hidenori Ichijo

    Article

    Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

    Inserts

    NRAS (NRAS Human)
    ASK1
    

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. N-BLa 1.1

    Plasmid

    #88994

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.1 fusion protein
    

    Tags

    Flag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. EYFP-NLS-actin-K68/284R

    Plasmid

    #60616

    Purpose

    Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

    Depositor

    Primal de Lanerolle

    Article

    Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    K68R, 284R

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pFAB3833

    Plasmid

    #47839

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 8 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1 p18 mito

    Plasmid

    #26638

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    p18 mito (LAMTOR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pXPR_BRD003 sgKEAP1-2

    Plasmid

    #74986

    Purpose

    sgKEAP1-2, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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