Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,047 results for: pET

Clear keywords
Showing: 5881 - 5900 of 10047 results

  1. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71699-plasmid-map-sequence-id-137034

    Enlarge

  2. Fast-GCaMP-EF20

    Plasmid
    #52645
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Publication
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fast-GCaMP-EF20
    Expression
    Mammalian
    Mutation
    D362N/D366N mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  3. pcDNA3.1(+)-RasAR-NES

    Plasmid
    #205567
    Purpose
    Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.
    Depositor
    Jin Zhang
    Publication
    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RasAR-NES (RAF1 Synthetic, Human)
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205567-plasmid-map-sequence-id-409398

    Enlarge

  5. SUV39H1

    Plasmid
    #28113
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H1 (SUV39H1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains chromo domain only (amino acid residues …
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  6. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

    Enlarge

  7. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72391-plasmid-map-sequence-id-133988

    Enlarge

  8. FKBP8

    Plasmid
    #25609
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FKBP8 (FKBP8 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    FKBP-type PPIase Domain, residues 90-205
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25609-plasmid-map-sequence-id-454823

    Enlarge

  11. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105771-plasmid-map-sequence-id-203360

    Enlarge

  13. pSUMO-YHRC

    Plasmid
    #54336
    Purpose
    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.
    Depositor
    Claes Andréasson
    Publication
    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d
    Type
    Empty backbone
    Tags
    6His-SUMO (yeast gene: SMT3)
    Expression
    Bacterial
    Promoter
    T7-LacO
    Available since
    Aug. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54336-plasmid-map-sequence-id-84530

    Enlarge

  14. mApple-CD151-7

    Plasmid
    #54873
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD151 (CD151 Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54873-plasmid-map-sequence-id-336040

    Enlarge

  16. 6BHD (SETDB1)

    Plasmid
    #119955
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1 (SETDB1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 190-410
    Promoter
    T7
    Available since
    Feb. 1, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119955-plasmid-map-sequence-id-234347

    Enlarge

  18. RAB26

    Plasmid
    #25528
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB26 (RAB26 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. HECW1

    Plasmid
    #25347
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    hecw1.0900.1035 (HECW1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5881 - 5900 of 10047 results