We narrowed to 43,166 results for: cha

1. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. hRIP1 HA GFP K45A

   Plasmid

   #41389

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP1 (RIPK1 Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (K45A)

   Promoter

   CMV

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. CaM/pIRES2-eGFP

   Plasmid

   #111499

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   calmodulin [Xenopus laevis] (calm2 Frog)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_TRE_Z17-PDGFR_EF1a_mCherry

   Plasmid

   #186303

   Purpose

   Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   Z17-PDGFR
   mCherry
   

   Use

   Tags

   HA, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

   Plasmid

   #79373

   Purpose

   Recruits VPR to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KG#121

   Plasmid

   #110879

   Purpose

   Expresses the C. elegans unc-31 cDNA pan-neuronally

   Depositor

   Kenneth Miller

   Article

   Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

   Inserts

   rab-3 promoter
   unc-31 cDNA
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Missing first 408bp

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB22

   Plasmid

   #122244

   Purpose

   Lentivirus delivery for stable expression of AsCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRMTK-clo6

   Plasmid

   #203957

   Purpose

   Cloning plasmid containing the kanamycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Kanamycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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