We narrowed to 6,088 results for: pcDNA 3.1

1. BE3-NLS-NLS-P2A-BLAST

   Plasmid

   #135348

   Purpose

   Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   BE3-2XNLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTE4566

   Plasmid

   #88904

   Purpose

   Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   hMbCpf1(D986A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TrpV1 scFv [N221/17]

   Plasmid

   #182082

   Purpose

   Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)
   

   Tags

   Sortase, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Myc-Nix-CytoB5

   Plasmid

   #100756

   Purpose

   Mammalian expression of ER targeted Nix

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   Nix-CytoB5 (BNIP3L Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TM domain replaced by CytoB

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mVenus-proα2(I)

   Plasmid

   #119838

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TMEM138-mCherry

   Plasmid

   #41630

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pTBL472

   Plasmid

   #126457

   Purpose

   Expresses Cas9-10xFlag-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   10x Flag
   TdT (DNTT Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCerulean-proα2(I)

   Plasmid

   #119836

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRT1-His-H2B-Cherry-2A (IG156)

   Plasmid

   #99622

   Purpose

   To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   His-H2B-Cherry
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-Mm_MyoGEF(WT)

    Plasmid

    #136325

    Purpose

    Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGAL4-DBD-HOXA13-58A-IDR

    Plasmid

    #145238

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-58A-IDR (HOXA13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-cCGNL1-Myc

    Plasmid

    #236516

    Purpose

    For expression of Paracingulin (dog) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGNL1 (CGNL1 Canis lupus familiaris (dog))
    

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MPC1-AA-FLAG

    Plasmid

    #228904

    Purpose

    Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tag

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K45 and K46 motif sites were both replaced with a…

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CIRC#del

    Plasmid

    #186344

    Purpose

    Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-SKP1-K142R

    Plasmid

    #213423

    Purpose

    Expresses human SKP1 K142R mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-K142R (SKP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-SKP1-97-101-5A

    Plasmid

    #213642

    Purpose

    Expresses human SKP1-97-101-5A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1--97-101-5A (SKP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag-SKP1-K163R

    Plasmid

    #213424

    Purpose

    Expresses human SKP1 K163R mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-K163R (SKP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. anti-GFAP R416WT scFv [N206B/9] with sortase tag

    Plasmid

    #204420

    Purpose

    Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
    

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSI-218

    Plasmid

    #197139

    Purpose

    All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SpCas9-PmCDA1-UGI

    Expression

    Mammalian

    Mutation

    SpCas9(D10A)

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge