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JAB2283

Plasmid

#190364

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ZAT10(RD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ZAT10(RD)-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-Erg11-iGFPx3

Plasmid

#115422

Purpose

Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Erg11-iGFPx3

Tags

iGFP

Expression

Yeast

Promoter

Endogenous

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CHIKV-nsP3-c001

Plasmid

#173086

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

N/A

Tags

mCerulean

Expression

Bacterial

Mutation

A1334-T1493, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo

Plasmid

#115420

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(203)

Plasmid

#44377

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(203)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pZCAN

Plasmid

#129115

Purpose

Z. rouxii centromeric vector containing ClonNAT resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*NoBody

Plasmid

#162807

Purpose

STELLA plasmid for amber suppression of N-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)NoBody

Use

Piggybac

Expression

Mammalian

Mutation

NoBody (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL

Plasmid

#23103

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pGSTag Bim EL (Bcl2l11 Mouse)

Tags

GSTag

Expression

Bacterial

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pUC19/mlcR25-mEGFP

Plasmid

#154295

Purpose

Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP

Expression

Bacterial

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-prp16-302

Plasmid

#89974

Purpose

prp16-302 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

prp16-302 allele (R456K and G691R)

Available Since

Aug. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFThGW

Plasmid

#27228

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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hSgk1_v3 Flag

Plasmid

#86039

Purpose

full length human Sgk1_i3 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2012 Dec 1;3

Insert

Alternate transcript v3 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v3; S313G

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1 (M3)

Plasmid

#45896

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 M3 mutant (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M3 (W229R) in SH3 domain

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF

Plasmid

#44374

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJMH024

Plasmid

#186636

Purpose

aadA.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B20-Leum

Plasmid

#44325

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B20-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B20-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pZEN

Plasmid

#129113

Purpose

Z. rouxii episomal vector containing ClonNAT resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pZCAG

Plasmid

#129114

Purpose

Z. rouxii centromeric vector containing G418 resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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