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pME-MLS-KillerRed

Plasmid

#115517

Purpose

Middle entry plasmid containing membrane bound KillerRed fluorophore

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Expression

Mutation

Promoter

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mTagBFP2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pME EFS-rtTA/rtTA3 (JDW 1230)

Plasmid

#224525

Purpose

A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

EFS-rtTA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-mmCdh5pro (JDW 1239)

Plasmid

#224478

Purpose

Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Cdh5 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

Plasmid

#232127

Purpose

A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA1 (GATA1 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE4

Plasmid

#195369

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…

Tags

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR8A L3-L2

Plasmid

#158735

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR5A L3-L2

Plasmid

#158732

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF8A L1-L4

Plasmid

#158731

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF5A L1-L4

Plasmid

#158728

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE5

Plasmid

#195371

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x8-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

Tags

5' and 3' NLS

Expression

Plant

Mutation

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR6A L3-L2

Plasmid

#158733

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR7A L3-L2

Plasmid

#158734

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF7A L1-L4

Plasmid

#158730

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NI)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF6A L1-L4

Plasmid

#158729

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p5E TRE-3GV (JDW 1163)

Plasmid

#224476

Purpose

Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

TRE3GV promoter

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_short (JDW 1046)

Plasmid

#224480

Purpose

Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

min-ICAM2 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_BFP

Plasmid

#25891

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

BFP

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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