We narrowed to 10,132 results for: yeast

1. pDONR TDP43 WT YFP

   Plasmid

   #27470

   Depositor

   Aaron Gitler

   Article

   Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC

   Plasmid

   #135465

   Purpose

   Membrane-anchored TF used for yeast directed evolution of TEV protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
   

   Tags

   BFP, flag

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LOV-Turbo-V5_pRS415

   Plasmid

   #199667

   Purpose

   expresses LOV-Turbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Tags

   V5

   Expression

   Yeast

   Promoter

   Gal1,10

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AU1-mTOR-E2419K

   Plasmid

   #19994

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-E2419K (Mtor Rat)
   

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Glutamine 2419 to Lysine

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-yomRuby3-SpHIS5

   Plasmid

   #204287

   Purpose

   For C-terminal tagging using yeast optimized mRuby3

   Depositor

   Linda Huang

   Article

   Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

   Insert

   pFA6-yomRuby3-SpHIS5
   

   Tags

   yomRuby3

   Expression

   Yeast

   Promoter

   none

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-puro-Venus-FLAG-CD59

   Plasmid

   #50377

   Purpose

   Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Article

   Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

   Insert

   Venus
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. BAS286 C terminal SF-GFP hygBR

   Plasmid

   #74081

   Purpose

   S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   super-folder GFP
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDuRCC-N

   Plasmid

   #81194

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please view depositor comments below and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB1_12_pGAP

   Plasmid

   #98498

   Purpose

   BB1 with insert: promoter PGAP

   Depositor

   Diethard Mattanovich, Brigitte Gasser, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   GAP promoter
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-AU1-mTOR-L1460P

    Plasmid

    #19995

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-L1460P (Mtor Rat)
    

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Leucine 1460 to Proline

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCDFDuet-trAtCCD7-OsD27(pYL726 )

    Plasmid

    #178285

    Purpose

    Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    OsD27
    trAtCCD7
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJZC603

    Plasmid

    #62317

    Purpose

    sgRNA with 2x PP7 for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x PP7 RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIMVAd40R

    Plasmid

    #98309

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1

    Expression

    Yeast

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-neo-HA-hApg12

    Plasmid

    #22950

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

    Insert

    autophagy related genes 12 (ATG12 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWL89A mmPing20

    Plasmid

    #145788

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20
    

    Expression

    Yeast

    Mutation

    T162C, T258A, T287C, T300A, T304A, T310A, G372A

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAN147

    Plasmid

    #129202

    Purpose

    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-mTIP49a

    Plasmid

    #15357

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pJZC545

    Plasmid

    #62313

    Purpose

    sgRNA with 1x MS2 for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x MS2 binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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