161,152 results

1. TFORF1166

   Plasmid

   #143471

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EBF4 (EBF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2-autophagosome-lysosome reporters

   Plasmid

   #196135

   Purpose

   zebrafish expression

   Depositor

   Reinhard Köster

   Insert

   autophagosome-lysosome reporters
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-nCFP

   Plasmid

   #185360

   Purpose

   For mammalian expression of N-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-cCFP

   Plasmid

   #185361

   Purpose

   For mammalian expression of C-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.UBI.GFP.miR292T

   Plasmid

   #115972

   Purpose

   miR-292 regulated GFP expression

   Depositor

   Johan Jakobsson

   Article

   Sachdeva et al Proc Natl Acad Sci U S A. 2010 Jun 22;10

   Insert

   GFP.miR292T
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   ubiquitin

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIK3C2A gRNA (BRDN0001149181)

   Plasmid

   #76414

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PIK3C2A gRNA (BRDN0001148547)

   Plasmid

   #76415

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IB-pSu9-HaloTag7-mGFP

   Plasmid

   #184905

   Purpose

   Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   Presequence of Fo-ATPase subunit 9
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOBI-FLuc

   Plasmid

   #170674

   Purpose

   Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   luc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBbi-RN

    Plasmid

    #60519

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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