We narrowed to 7,234 results for: CAR

Showing: 5941 - 5960 of 7234 results

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gRNA_SOX2_HDR

Plasmid

#163752

Purpose

gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

Depositor

Jennifer Mitchell

Publication

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SOX2 (SOX2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEGGsH6-MCS_SHORT_inFrame

Plasmid

#211438

Purpose

Vector backbone for random polymerase chain reaction screen

Depositor

Helmut Pospiech

Insert

MCS-tev cleavage site-EGFP-6xHis-Tag

Expression

Bacterial

Available since

March 1, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 E2E1- Prg4 promoter-luciferase

Plasmid

#195035

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)

Use

Luciferase

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.10 E3E2E1-Prg4 promoter-luciferase

Plasmid

#195036

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)

Use

Luciferase

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

Plasmid

#195037

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)

Use

Luciferase

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2_172

Plasmid

#199104

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2461

Plasmid

#190368

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-IcaRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Publication

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-IcaRO::GFPint-NLS-ADHt

Expression

Plant

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDx_FusionRed-SYFP2

Plasmid

#189760

Purpose

Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

FusionRed-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5b_Ptet

Plasmid

#179338

Purpose

gRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a_Ptet

Plasmid

#179337

Purpose

gRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRS426-PGAL7-A_GFP_TCYC1

Plasmid

#177162

Purpose

Expresses yeGFP under the control of a synthetic promoter (PGAL7-A)

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeGFP

Expression

Yeast

Promoter

Synthetic promoter (PGAL7-A)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-B_GFP_TCYC1

Plasmid

#177163

Purpose

Expresses yeGFP under the control of a synthetic promoter (PGAL7-B)

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeGFP

Expression

Yeast

Promoter

Synthetic promoter (PGAL7-B)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-D_RFP_TCYC1

Plasmid

#177165

Purpose

Expresses yeRFP under the control of a synthetic promoter (PGAL7-D)

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeCherry

Expression

Yeast

Promoter

Synthetic promoter (PGAL7-D)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pmx-puro-Mef2c-i

Plasmid

#172398

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Publication

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i (Mef2c Mouse)

Use

Retroviral

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAshort

Plasmid

#153547

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Publication

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 603-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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sYFP2-OP18-mScI

Plasmid

#128003

Purpose

Redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

Depositor

Dorus Gadella

Publication

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

sYFP2-OP18-mScI

Expression

Mammalian

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1948

Plasmid

#154340

Purpose

Enhancer reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz106_CsoS2_MR

Plasmid

#140843

Purpose

His-tagged CsoS2 Middle Region (MR), pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 Middle Region (MR)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

only amino acids 257-603

Promoter

T7

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Publication

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.34

Plasmid

#131370

Purpose

expression of GST:MEG-4 in C. elegans

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

meg-4 (C36C9.1 Nematode)

Expression

Bacterial

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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