Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,040 results for: SIM

Showing: 5941 - 5960 of 7040 results

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mOrange-imp α

Plasmid

#119720

Purpose

Expresses mOrange-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

mOrange

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-RSLID1(WT)

Plasmid

#122296

Purpose

expresses RSL1D1 protein in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

vector's LTR

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C

Plasmid

#114629

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 49 polyQ repeat

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

Plasmid

#114615

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 23 polyQ repeat

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

Plasmid

#114628

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

Plasmid

#114621

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 37 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

Plasmid

#114614

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 23 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

Plasmid

#114624

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

Plasmid

#114625

Purpose

Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

Plasmid

#114630

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

Plasmid

#114610

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

Plasmid

#114609

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

Plasmid

#114620

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

Plasmid

#114619

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

Plasmid

#114618

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

Plasmid

#114608

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 15 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

Plasmid

#114612

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C; 23 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLuc-Optop38i-dsm

Plasmid

#89748

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-APEX1

Plasmid

#215126

Purpose

Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX1 (APEX1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-hMLH1dn

Plasmid

#222997

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Deletion of residues 754-756 in MLH1 as described…

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 5941 - 5960 of 7040 results

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