We narrowed to 16,128 results for: form

1. pSUP-(2xCouRS-tRNAopt)

   Plasmid

   #197930

   Purpose

   Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

   Inserts

   CouRS
   tRNA opt
   

   Expression

   Bacterial

   Promoter

   glnS' and lambda(ts) and proK

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAT7

   Plasmid

   #204292

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Caspase-Reporter

   Plasmid

   #243673

   Purpose

   Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

   Depositor

   Matthias Wirth

   Article

   Selcen et al Cell Death Discov, 11, 368, 06 August 20

   Insert

   Split-GFP with DEVD sequence and T2A mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-LacI

   Plasmid

   #179510

   Purpose

   Expresses EGFP-LacI in mammalian cells

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   LacI
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EWB-DIO-cyan pre-eGRASP(p32)

   Plasmid

   #111589

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p32)
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 CARD8 Ub-S297

   Plasmid

   #169965

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Ub

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJA167

   Plasmid

   #128536

   Purpose

   Expresses DsRed

   Depositor

   Marcos Alcocer

   Article

   Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

   Insert

   NFAT-DsRed reporter
   

   Expression

   Mammalian

   Promoter

   SV40-NFAT

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-FOXO1A

   Plasmid

   #153141

   Purpose

   Expression of Flag tagged human FOXO1A in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXO1 (FOXO1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB512

    Plasmid

    #74854

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLyGo-Ec-8

    Plasmid

    #163136

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P(rhaB)

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLP-hyg

    Plasmid

    #87831

    Purpose

    Expresses FLP recombinase with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    heat-shock inducible

    Available Since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

    Plasmid

    #111581

    Purpose

    An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myrTagRFP-T-P2A-post-eGRASP
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-191084-gp160

    Plasmid

    #123234

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Sst44-GFP

    Plasmid

    #172310

    Purpose

    SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst44
    

    Use

    AAV

    Promoter

    pB-Globin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. actc1b-mKate2-P2A-APEX2-VHH[GFP]

    Plasmid

    #126749

    Purpose

    Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    VHH[EGFP]
    

    Use

    Zebrafish muscle expression clone

    Tags

    mKate2-P2A-APEX2

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-FOXC1

    Plasmid

    #153114

    Purpose

    Expression of Flag tagged human FOXC1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXC1 (FOXC1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG_mRuby2_smFP FLAG

    Plasmid

    #59760

    Purpose

    encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    mRuby2_smFP_FLAG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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