We narrowed to 10,395 results for: plasmids 101

1. pIW086

   Plasmid

   #134132

   Purpose

   Bacterial expression of His-TEV-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-TEV-human Ska1 ; human Ska2
   

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K183A K184A

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.13/GUG-FFLuc-3XFLAG

   Plasmid

   #127334

   Purpose

   Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   Firefly luciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA3_MYOD1

   Plasmid

   #64138

   Purpose

   Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_MYOD1 (MYOD1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPC027.306

   Plasmid

   #171149

   Purpose

   Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ133D2.0

   Plasmid

   #99894

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ131D2.0

   Plasmid

   #100044

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSECB

   Plasmid

   #106305

   Purpose

   Express spCas9 with sgRNA, blast resistance

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.2 mir-1-1 reporter dme-mir-125

   Plasmid

   #46667

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-mir-125
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. gh174

   Plasmid

   #106835

   Purpose

   expression of gRNA targeting UGT8

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   UGT8 (UGT8 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2 mir-1-1 reporter mir30-wt

    Plasmid

    #46695

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir30-wt (MIR30A Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO-PBG-WPRE-hGH

    Plasmid

    #74287

    Purpose

    pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

    Insert

    PBG (chimeric rabies Glycoprotein)
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLP857_α-syn:oScarlet KI donor

    Plasmid

    #239403

    Purpose

    AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

    Depositor

    Subhojit Roy

    Article

    Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

    Insert

    oScarlet
    

    Use

    AAV and CRISPR

    Tags

    oScarlet

    Expression

    Mammalian

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZNpF-∆7

    Plasmid

    #245367

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆7 (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    7-bp deletion, H96Vfs*29

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZNpF-∆8

    Plasmid

    #245368

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆8 (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    synthetic 8-bp deletion

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNpF-∆59

    Plasmid

    #245365

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆59 (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    59-bp deletion, D78Vfs*6

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZNpF-∆11

    Plasmid

    #245366

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆11 (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    11-bp deletion, H96Wfs*4

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-DIO-TurboID

    Plasmid

    #237885

    Purpose

    AAV vector for Cre-dependent expression of TurboID

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    TurboID
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-DIO-seTurboID

    Plasmid

    #237886

    Purpose

    AAV vector for Cre-dependent expression of seTurboID

    Depositor

    Rui Lin

    Article

    Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

    Insert

    seTurboID
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221838

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221839

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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