3,636 results

1. pMesh1*

   Plasmid

   #175591

   Purpose

   Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Mesh1 (Mesh1 Fly)
   

   Tags

   Fused with a flexible GS linker to cerulean

   Expression

   Bacterial

   Mutation

   Codon optimized for translation in E.coli. AGGAGG…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LAMP1-GFP

   Plasmid

   #16290

   Depositor

   Ron Vale

   Article

   Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

   Insert

   LAMP1/RE72002
   

   Tags

   EGFP

   Expression

   Insect

   Promoter

   Ac5

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASTattB-GFP-Msp300KASH

   Plasmid

   #170806

   Purpose

   Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

   Depositor

   Vikki Weake

   Article

   Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

   Insert

   GFP-Msp300KASH
   

   Tags

   GFP

   Expression

   Insect

   Promoter

   5xUAS + hsp70 minimal promoter

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACUH-GFP11x7-mCherry-α-tubulin

   Plasmid

   #70218

   Purpose

   Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11x7-mCherry-α-tubulin
   

   Tags

   GFP11x7 and mCherry

   Expression

   Insect

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217515

   Purpose

   Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. KHBD00727

   Plasmid

   #39748

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG9932 (CG9932 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1(+) Laccase2 MCS Exon Vector

   Plasmid

   #69893

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   Laccase2 (stw Fly)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRE-Hsp70BbCas9_1.3

   Plasmid

   #190797

   Purpose

   aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCiB-yin

   Plasmid

   #13845

   Depositor

   Ting Wu

   Article

   Bateman et al Genetics. 2006 Jun . 173(2):769-77.

   Insert

   yellow (y Fly)
   

   Expression

   Insect

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT-ADARcd-E488Q-V5

    Plasmid

    #139686

    Purpose

    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Adenosine deaminase acting on RNA (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PRExpress

    Plasmid

    #122486

    Purpose

    A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Inserts

    LacZ
    poly-PRE
    

    Expression

    Insect

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJFRC12-10XUAS-IVS-myr::GFP

    Plasmid

    #26222

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    myr::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASTP2.1

    Plasmid

    #13842

    Depositor

    Ting Wu

    Article

    Bateman et al Genetics. 2006 Jun . 173(2):769-77.

    Insert

    mini-white (w Fly)
    

    Expression

    Insect

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. nSYB-GAL80-DD

    Plasmid

    #124778

    Purpose

    Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GAL80 (GAL80-DD)
    

    Tags

    DD

    Expression

    Insect

    Promoter

    nSynaptobrevin

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 13XLexAop2 IVS ChrimsonR mCherry_tr

    Plasmid

    #111548

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
    

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Available Since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

    Plasmid

    #159630

    Purpose

    AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

    Depositor

    Andrew Allen, Ross Bathgate

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    Ast (AstA Fly)
    

    Use

    AAV

    Tags

    HA, IRES, mCherry, and neurophysin-1

    Expression

    Mammalian

    Promoter

    DCA (same as CAG promoter)

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

    Plasmid

    #69905

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (HIPK3 Human, Fly)
    

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQUAST

    Plasmid

    #24349

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    5xQUAS-Pmin promoter
    

    Expression

    Insect

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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