3,636 results

1. 5XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111545

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChrimson_tdtomato_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ovoD1 D1B2NR (18 kb)

   Plasmid

   #37280

   Depositor

   Norbert Perrimon

   Article

   Chou et al Development. 1993 Dec;119(4):1359-69.

   Insert

   ovoD1 genomic DNA fragment
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. SCP1-pUC119

   Plasmid

   #21846

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP1 (super core promoter 1)-pUC119
   

   Use

   Puc derivative - a cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LAMP1-GFP

   Plasmid

   #16290

   Depositor

   Ron Vale

   Article

   Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

   Insert

   LAMP1/RE72002
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC12-10XUAS-IVS-myr::GFP

   Plasmid

   #26222

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   myr::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pACUH-GFP11x7-mCherry-α-tubulin

   Plasmid

   #70218

   Purpose

   Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11x7-mCherry-α-tubulin
   

   Use

   Tags

   GFP11x7 and mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWC2

   Plasmid

   #15960

   Depositor

   Jeff Gelles

   Article

   Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

   Insert

   Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
   

   Use

   Tags

   BCCP and His6

   Expression

   Bacterial

   Mutation

   amino acids 1-401

   Promoter

   Available Since

   Nov. 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASP dsRed Rab7 WT

   Plasmid

   #44824

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab7 (Rab7 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+) Laccase2 MCS Exon Vector

    Plasmid

    #69893

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Rab9

    Plasmid

    #31736

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab9 (Rab9 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCFD3-ebony

    Plasmid

    #83380

    Purpose

    Drosophila gRNA expression plasmid targets ebony

    Depositor

    Richard Padgett

    Article

    Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

    Insert

    ebony (e Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKC31

    Plasmid

    #226777

    Purpose

    Expresses Drosophila UNC-104(R562H) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R562H) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 562 to Histidine (bris mutation)

    Promoter

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-MXC

    Plasmid

    #37140

    Depositor

    Robert Duronio

    Article

    White et al J Cell Biol. 2011 May 16;193(4):677-94.

    Insert

    Multi Sex combs (mxc Fly)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pQUAST

    Plasmid

    #24349

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    5xQUAS-Pmin promoter
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUASTattB-2xFlag-mCherry-Msp300KASH

    Plasmid

    #170807

    Purpose

    Expresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesis

    Depositor

    Vikki Weake

    Article

    Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

    Insert

    2xFLAG/mCherry-Msp300KASH
    

    Use

    Tags

    2xFLAG-mCherry and Msp300KASH

    Expression

    Insect

    Mutation

    Promoter

    5xUAS + hsp70 minimal promoter

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p026 pCS2-EnR

    Plasmid

    #11028

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    engrailed (en Fly)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Engrailed repressor domain (bases encoding amino …

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge