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Plasmid#225502PurposeInducible expression of FLAG-tagged mutated p37 proteinDepositorInsertp37 (UBXN2B Human)
UseLentiviral; Gateway destination vector, doxycycli…TagsFLAGExpressionMammalianMutationF215A/E218A/QKL220-222AAAPromoterTRE promoter, Tet ONAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-p37-WT-FLAG
Plasmid#225501PurposeInducible expression of FLAG-tagged wildtype p37 proteinDepositorInsertp37 (UBXN2B Human)
UseLentiviral; Gateway destination vector, doxycycli…TagsFLAGExpressionMammalianMutationnoPromoterTRE promoter, Tet ONAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97deltaD2
Plasmid#17229DepositorInsertp97 (Vcp Mouse)
UseTagsHisExpressionBacterialMutationchanged codon 457 to stop codonPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-UBE4B
Plasmid#113499PurposeExpression of human UBE4B with N-terminal GFP tagDepositorInsertUBE4B (UBE4B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5FRT/TO-UBXD3-Strep-HA
Plasmid#113488PurposeExpression of human UBXD3 with C-terminal strep-HA tagDepositorInsertUBXD3 (UBXN10 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD4-Strep-HA
Plasmid#113480PurposeExpression of human UBXD4 with C-terminal strep-HA tagDepositorInsertUBXD4 (UBXN2A Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p37-Strep-HA
Plasmid#113485PurposeExpression of human p37 with C-terminal strep-HA tagDepositorInsertp37 (UBXN2B Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-RNF185-GFP
Plasmid#113498PurposeExpression of human RNF185 with C-terminal GFP tagDepositorInsertRNF185 (RNF185 Human)
UseTagsGFPExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-HOIP-Strep-HA
Plasmid#113494PurposeExpression of human HOIP with C-terminal strep-HA tagDepositorInsertHOIP (RNF31 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-SAKS1-Strep-HA
Plasmid#113487PurposeExpression of human SAKS1 with C-terminal strep-HA tagDepositorInsertSAKS1 (UBXN1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PLAA
Plasmid#85669PurposeExpression of PLAA with N-terminal GFP tagDepositorInsertPLAA (PLAA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p47-Strep-HA
Plasmid#113475PurposeExpression of human p47 with C-terminal strep-HA tagDepositorInsertp47 (NSFL1C Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD7-Strep-HA
Plasmid#113479PurposeExpression of human UBXD7 with C-terminal strep-HA tagDepositorInsertUBXD7 (UBXN7 Human)
UseTags2xstrep-HAExpressionMammalianMutationA834C silent mutation, wildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Cezanne-Strep-HA
Plasmid#113492PurposeExpression of human Cezanne with C-terminal strep-HA tagDepositorInsertCezanne (OTUD7B Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-PNGase-Strep-HA
Plasmid#113490PurposeExpression of human PNGase with C-terminal strep-HA tagDepositorInsertPNGase (NGLY1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-PLAA-Strep-HA
Plasmid#113484PurposeExpression of human PLAA with C-terminal strep-HA tagDepositorInsertPLAA (PLAA Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-DVC1-Strep-HA
Plasmid#113481PurposeExpression of human DVC1 with C-terminal strep-HA tagDepositorInsertDVC1 (SPRTN Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-YOD1-Strep-HA
Plasmid#113483PurposeExpression of human YOD1 with C-terminal strep-HA tagDepositorInsertYOD1 (YOD1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD1-Strep-HA
Plasmid#113476PurposeExpression of human UBXD1 with C-terminal strep-HA tagDepositorInsertUBXD1 (UBXN6 Human)
UseTags2xstrep-HAExpressionMammalianMutationC174T silent mutation, wildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-AIRAPL-Strep-HA
Plasmid#113489PurposeExpression of human AIRAPL with C-terminal strep-HA tagDepositorInsertAIRAPL (ZFAND2B Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD5-Strep-HA
Plasmid#113493PurposeExpression of human UBXD5 with C-terminal strep-HA tagDepositorInsertUBXD5 (UBXN11 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Gal3
Plasmid#85662PurposeFluorescent reporter for lysosomal damageDepositorInsertLGALS3 (LGALS3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Npl4-Strep-HA
Plasmid#113495PurposeExpression of human Npl4 with C-terminal strep-HA tagDepositorInsertNpl4 (NPLOC4 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Ufd1-Strep-HA
Plasmid#113474PurposeExpression of human Ufd1 with C-terminal strep-HA tagDepositorInsertUfd1 (UFD1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1
Plasmid#85664PurposeExpression of human YOD1 with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD8-Strep-HA
Plasmid#113477PurposeExpression of human UBXD8 with C-terminal strep-HA tagDepositorInsertUBXD8 (FAF2 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-FAF1-Strep-HA
Plasmid#113486PurposeExpression of human FAF1 with C-terminal strep-HA tagDepositorInsertFAF1 (FAF1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-YOD1
Plasmid#85663PurposeExpression of human YOD1 with N-terminal mCherry tagDepositorInsertYOD1 (YOD1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-C160S
Plasmid#85666PurposeExpression of human YOD1 C160S mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationC160SPromoterCMVAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2-C160S
Plasmid#85668PurposeExpression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationC160S and I292Q and V295QPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2
Plasmid#85667PurposeExpression of human YOD1 I292Q V295Q mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationI292Q and V295QPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-VCF1 WT
Plasmid#223018PurposeExpression of GFP-tagged VCF1 in mammalian cells.DepositorInsertVCF1 (VCF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV/TetO2Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFL His-p97
Plasmid#113510PurposeExpression of His-p97 in insect cells (Baculovirus system)DepositorInsertp97 (VCP Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Strep-HA-VCF1 WT
Plasmid#223020PurposeExpression of Strep-HA-tagged VCF1 in mammalian cells.DepositorInsertVCF1 (VCF1 Human)
UseTagsStrep-HAExpressionMammalianMutationPromoterCMV/TetO2Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Ataxin3-Strep-HA
Plasmid#113496PurposeExpression of human Ataxin-3 with C-terminal strep-HA tagDepositorInsertAtaxin-3 (ATXN3 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-VCF1 N167A
Plasmid#223019PurposeExpression of GFP-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.DepositorInsertVCF1 (VCF1 Human)
UseTagsGFPExpressionMammalianMutationN167APromoterCMV/TetO2Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Strep-HA-VCF1 N167A
Plasmid#223021PurposeExpression of Strep-HA-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.DepositorInsertVCF1 (VCF1 Human)
UseTagsStrep-HAExpressionMammalianMutationPromoterCMV/TetO2Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
4LG1
Plasmid#60092PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21D:JMC072-C04:C227980 (VCPKMT Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralTagsExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b His-p97
Plasmid#169020PurposeExpresses His-p97 in bacteriaDepositorInsertp97
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeTagsExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-TEV-yVms1(1-417,delta(38-69))
Plasmid#128739PurposeBacterial overexpression and crystallizationDepositorInsertVms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
UseTagsGSTExpressionBacterialMutationresidues 1-417 with deletion of 38-69PromoterT7Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-His D592Amb
Plasmid#169022PurposeExpresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteriaDepositorInsertp97
UseTagsHisExpressionBacterialMutationD592TAGPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-Strep-His E314Amb
Plasmid#169021PurposeExpresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteriaDepositorInsertp97
UseTagsHis and SBPExpressionBacterialMutationE314TAGPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 *VBM
Plasmid#89976PurposeExpresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motifDepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationmutated 282RKRR to 282HNHHPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MATR3
Plasmid#32880DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only