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Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationRed mutant S286YPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 GFP-Atl1
Plasmid#86679PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorInsertATL1 (ATL1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL1
Plasmid#109018Purposestable lentiviral expression of cDNADepositorInsertATL1 (ATL1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p4xSMAD_RE
Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-myr-mTagBFP2-Flag (JDW 1183)
Plasmid#224492PurposeGateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)DepositorInsertmyr-mTagBFP2-Flag
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-MED1LCD
Plasmid#204404PurposeE. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tagDepositorInsertMED1(947-1581) (MED1 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL1-5
Plasmid#109007PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL1-sgRNA #5 (ATL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-Myc-ATL1-oPRE
Plasmid#233471PurposeAAV vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertATL1 (ATL1 Human)
UseAAVTagsmyc-tagExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL1-2
Plasmid#109006PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL1-sgRNA #2 (ATL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL1-G20TM
Plasmid#109019Purposestable lentiviral expression of cDNADepositorInsertATL1-deltaTM (ATL1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_CELF2
Plasmid#106102PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2DepositorInsertgRNA targeting CELF2 (CELF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FB026
Plasmid#119711PurposeTranscriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPromoter gpdA:YFP coding sequence:Terminator trpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZL hygro Flag HA TRAP220 wt
Plasmid#17433PurposeRetroviral expression of Med1/TRAP220 in mammalian cellsDepositorInsertTRAP220 (MED1 Human)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR-MED1trunc-IDR-YFP-NLS
Plasmid#145275PurposeMammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLSDepositorInsertMED1-IDR truncated (MED1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-MED1long-IDR-YFP-NLS
Plasmid#145276PurposeMammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLSDepositorInsertMED1-IDR long (MED1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald
Plasmid#225944PurposeLentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
GLYCTK gRNA (BRDN0001487112)
Plasmid#77905Purpose3rd generation lentiviral gRNA plasmid targeting human GLYCTKDepositorInsertGLYCTK (GLYCTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLYCTK gRNA (BRDN0001147448)
Plasmid#77906Purpose3rd generation lentiviral gRNA plasmid targeting human GLYCTKDepositorInsertGLYCTK (GLYCTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLYCTK gRNA (BRDN0001146438)
Plasmid#77907Purpose3rd generation lentiviral gRNA plasmid targeting human GLYCTKDepositorInsertGLYCTK (GLYCTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLYCTK gRNA (BRDN0001145738)
Plasmid#77908Purpose3rd generation lentiviral gRNA plasmid targeting human GLYCTKDepositorInsertGLYCTK (GLYCTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-GLYCTK
Plasmid#23653DepositorInsertGLYCTK (GLYCTK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-mCherry-MED1-IDR
Plasmid#194545PurposeBacterial Expression of 6xHis mCherry-MED1-IDR Fusion ProteinDepositorInsertMED1 (MED1 Human)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only