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Showing: 41 - 60 of 169 results

  2. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161928-plasmid-map-sequence-id-325287

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  3. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161927
    Purpose
    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine repression reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161927-plasmid-map-sequence-id-324349

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  4. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188747
    Purpose
    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188747-plasmid-map-sequence-id-371444

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  6. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188743
    Purpose
    Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RSV
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188743-plasmid-map-sequence-id-371436

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  7. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188744
    Purpose
    Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188744-plasmid-map-sequence-id-371443

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  8. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188745
    Purpose
    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nonTATAchr21
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188745-plasmid-map-sequence-id-371435

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  9. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188746
    Purpose
    Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nonTATAchrX
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188746-plasmid-map-sequence-id-373413

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  10. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  11. pFUSE2ss-CLIg-mK_M18

    Plasmid
    #82358
    Purpose
    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    82358-plasmid-map-sequence-id-400982

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  13. A24M04 Ig kappa light chain-pFEEKW

    Plasmid
    #234810
    Purpose
    Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1
    Depositor
    Susan Pierce
    Article
    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12
    Insert
    A24M04 Igkappa light chain (IGKC, IGK Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EEK promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234810-plasmid-map-sequence-id-466812

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  14. pCMV-iGlu3Fast

    Plasmid
    #226500
    Purpose
    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release
    Depositor
    Katalin Torok
    Article
    Tran et al bioRxiv, August 30th, 2024
    Insert
    iGluSnFR3 S72T
    Use
    Tags
    IgK, Myc tag, and PDGFR Beta TM domain
    Expression
    Mammalian
    Mutation
    S72T
    Promoter
    CMV
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226500-plasmid-map-sequence-id-448688

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  15. pCMV-iGlu3slow1

    Plasmid
    #226501
    Purpose
    Superslow-decay iGluSnFR3 variant 1 for population imaging
    Depositor
    Katalin Torok
    Article
    Tran et al bioRxiv, August 30th, 2024
    Insert
    iGluSnFR3 D25A
    Use
    Tags
    IgK, Myc tag, and PDGFR Beta TM domain
    Expression
    Mammalian
    Mutation
    D25A
    Promoter
    CMV
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226501-plasmid-map-sequence-id-449138

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  16. pCMV-iGlu3slow2

    Plasmid
    #226502
    Purpose
    Superslow-decay iGluSnFR3 variant 2 for population imaging
    Depositor
    Katalin Torok
    Article
    Tran et al bioRxiv, August 30th, 2024
    Insert
    iGluSnFR3 D25R
    Use
    Tags
    IgK, Myc tag, and PDGFR Beta TM domain
    Expression
    Mammalian
    Mutation
    D25R
    Promoter
    CMV
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226502-plasmid-map-sequence-id-448687

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  17. 139H2_LC

    Plasmid
    #206202
    Purpose
    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.
    Depositor
    Joost Snijder
    Article
    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202
    Insert
    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    206202-plasmid-map-sequence-id-408742

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  18. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid
    #61883
    Purpose
    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61883-plasmid-map-sequence-id-358014

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  19. pVITRO1-Trastuzumab-IgG2/κ

    Plasmid
    #61884
    Purpose
    Expresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61884-plasmid-map-sequence-id-207869

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  20. pVITRO1-Trastuzumab-IgG4/κ

    Plasmid
    #61887
    Purpose
    Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61887-plasmid-map-sequence-id-101186

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Showing: 41 - 60 of 169 results