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Plasmid#65355PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtI-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorInsertLactate dehydrogenase (LDH Bovine, Bos taurus)
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-CDC19-CYC1t
Plasmid#216122PurposeYeast integrative vector for constitutive expression of CDC19 at the TRP1 locusDepositorInsertPyruvate kinase 1
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-Dimer-1SUMO
Plasmid#190249PurposeExpression dimer-1SUMO component in yeast cellsDepositorInsertdimer-SUMO
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-SUMO7-6SIM
Plasmid#190250PurposeExpression SUMO7-SIM6 in yeast cellsDepositorInsert7SUMO-6SIM
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
POT5-TDH3p-ycrtYB-ADH1t
Plasmid#65358PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-TDH3p-ycrtE-ADH1t
Plasmid#65352PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsbtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Ptdh3-ACDH-Trpl3
Plasmid#127746PurposeYeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV106-pTDH3-mCherry-10xSumo-6xSIM
Plasmid#116928PurposeYeast integrative vector for expression of the SUMO-SIM complexDepositorInsert10xSUMO-6xSIM
UseTagsmCherryExpressionYeastMutationPromoterTDH3Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-Elo3-GFP11-Ura
Plasmid#177734PurposeER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA markerDepositorInsertElo3 (ELO3 Budding Yeast)
UseTagsGFP11 and HDELExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP11-Ura
Plasmid#177731PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid withDepositorInsertFlucDM
UseTagsGFP11, HA, HDEL, and KAR2 Signal SequenceExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
UseTagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-MTS-GFP11-Ura
Plasmid#177733PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA markerDepositorInsertMTS-GFP11
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)
Plasmid#167357PurposeIntegrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1tDepositorInsertTDH3p:his6-mKate2-LipPks1-IDP1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)
Plasmid#167356PurposeIntegrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1tDepositorInsertTDH3p:his6-mKate2-MBP-IDP1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p403 – TDH3-MTS-mCherry-GFP1-10-His
Plasmid#177730PurposemCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS markerDepositorInsertmCherry-GFP1-10
UseTagsMitochondrial Targeting SequenceExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only