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Plasmid#117854PurposeExpressed KpAGO 207-1251 in E. coli under IPTG-inducible promotorDepositorInsertArgonaute
UseTags6xHis-SUMO encoded in vectorExpressionBacterialMutationmissing first 206 amino acid residuesPromoterT7Available sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-5xA
Plasmid#91979PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-WT
Plasmid#91986PurposeExpresses FLAG-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-5xE
Plasmid#91981PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2
Plasmid#10821DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsFLAG and HAExpressionMammalianMutationR668H. Additionally, N terminus different from wi…PromoterAvailable sinceOct. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387A
Plasmid#59905PurposeExpresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationS387A, phospho-null alaninePromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-S828A
Plasmid#91982PurposeExpresses FLAG-HA-AGO2 (S828A)DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationS828APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387E
Plasmid#59906PurposeExpresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationS387E, phospho-dominantPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2D-A
Plasmid#221734PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD683A in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2DY-EF
Plasmid#221733PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD651E and Y680F in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2Y-F
Plasmid#221732PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationY680F in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2D-E
Plasmid#221731PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD651E in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2
Plasmid#221730PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2 (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2EF-DY
Plasmid#221723PurposeExpression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2EF-DY (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D and F666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2F-Y
Plasmid#221722PurposeExpression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2F-Y (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationF666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2E-D
Plasmid#221721PurposeExpression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2E-D (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2D-A
Plasmid#221720PurposeExpression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2D-A (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationD669A in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2
Plasmid#221719PurposeExpression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2 (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationPromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2DY-EF
Plasmid#221716PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E and Y680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2Y-F
Plasmid#221715PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationY680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only