We narrowed to 687 results for: emd

1. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

   Plasmid

   #190237

   Purpose

   Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMtb CblA

   Plasmid

   #138298

   Purpose

   Expression of Mtb CblA

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Insert

   Mtb CblA
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMtb ATR

   Plasmid

   #138297

   Purpose

   Expression of Mtb ATR

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Insert

   Corrinoid adenosyl transferase
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNAP-MIDAS

   Plasmid

   #171604

   Purpose

   Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
   

   Use

   Tags

   His6 tag and SNAP tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET32a-hAREG

   Plasmid

   #199229

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Amphiregulin preproprotein (AREG Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-ybbr-HIS-ddFLN4-Fgß

   Plasmid

   #101719

   Purpose

   wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET19b-MED17

   Plasmid

   #15412

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

   Insert

   MED17 (MED17 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

10. ELF2_pet28a

    Plasmid

    #131662

    Purpose

    Bacterial expression of ETS gene ELF2

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF2 (ELF2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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