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Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
UseTagsN-term His6 tag and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTet
Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD
Plasmid#127785PurposeExpresses CtCHAD in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GRLF1
Plasmid#36195PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains the Ras homolog domain only (amino acid …PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
dFP
Plasmid#120542PurposeExpresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.DepositorInsertde novo fluorescence-activating protein (dFP)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-FL
Plasmid#127795PurposeExpresses full-length ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+GFP
Plasmid#233003PurposeGalactose iduced expression of Gcn4 WW+GFP in yeastDepositorInsertGcn4 WW+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+GFP
Plasmid#233001PurposeGalactose iduced expression of Gcn4 Aro2+GFP in yeastDepositorInsertGcn4 Aro2+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol
Plasmid#232976PurposeGalactose iduced expression of Gcn4 solvvol in yeastDepositorInsertGcn4 solvvol
UseTagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 S/ArotoL
Plasmid#232979PurposeGalactose iduced expression of Gcn4 S/ArotoL in yeastDepositorInsertGcn4 S/ArotoL
UseTagsTEV cleavage siteExpressionYeastMutationS101L, S104L, F108L, Y110L, S117L, S136L, S144LPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WHA
Plasmid#232995PurposeGalactose iduced expression of Gcn4 ILVtoED W+HAin yeastDepositorInsertGcn4 ILVtoED W+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only