3,686 results
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Plasmid#206483PurposeVasa expression for Schneider cell imagingDepositorInsertVasa
TagsEGFPExpressionInsectAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
ExpressionInsectAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
ExpressionInsectAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p Pcrg1: Khd4-Ada-Gfp (CbxR)
Plasmid#203734PurposePlasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, folDepositorInsertKhd4-Ada-Gfp
TagsGfpExpressionBacterialPromoterPcrg1Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH
Plasmid#148939PurposeInsect Expression of DmGIGYF-WFFtoAAADepositorInsertDmGIGYF-WFFtoAAA (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH
Plasmid#148909PurposeInsect Expression of Dm4EHP-W114ADepositorInsertDm4EHP-W114A (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP_AH
Plasmid#148914PurposeInsect Expression of Dm4EHPDepositorInsertDm4EHP (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP-W85A_AH
Plasmid#148917PurposeInsect Expression of Dm4EHP-W85ADepositorInsertDm4EHP-W85A (4EHP Fly)
ExpressionInsectMutation4 silent mutations compared to the sequence given…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_AH
Plasmid#148918PurposeInsect Expression of DmGIGYFDepositorInsertDmGIGYF (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_641-1574_AH
Plasmid#148920PurposeInsect Expression of DmGIGYF_641-1574DepositorInsertDmGIGYF_641-1574 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
ExpressionInsectMutation322-1666 truncated version of elF4G sequence with…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAtx2_AE
Plasmid#148548PurposeInsect Expression of DmAtx2DepositorInsertDmAtx2 (Atx2 Fly)
ExpressionInsectMutationThree silent mutations and two deletions compared…Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRpL10_R
Plasmid#147348PurposeInsect Expression of DmRpl10DepositorInsertDmRpl10 (RpL10 Fly)
ExpressionInsectAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorPublicationInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW249_Pzipt17-loxP-H2BGFP-miniMOS
Plasmid#196063Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.DepositorInsertsH2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR
ExpressionWormAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmMe31B_G
Plasmid#146381PurposeBacterial Expression of DmMe31BDepositorInsertDmMe31B (me31B Fly)
ExpressionBacterialAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-MBP-DmBam_13-36_AG
Plasmid#148839PurposeMammalian Expression of DmBam_13-36DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDmBam_13-36 (bam Fly)
ExpressionMammalianAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_2
Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR Bcd-LEXY
Plasmid#182594PurposeDrosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only