3,648 results
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Plasmid#148920PurposeInsect Expression of DmGIGYF_641-1574DepositorInsertDmGIGYF_641-1574 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
ExpressionInsectMutation322-1666 truncated version of elF4G sequence with…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAtx2_AE
Plasmid#148548PurposeInsect Expression of DmAtx2DepositorInsertDmAtx2 (Atx2 Fly)
ExpressionInsectMutationThree silent mutations and two deletions compared…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRpL10_R
Plasmid#147348PurposeInsect Expression of DmRpl10DepositorInsertDmRpl10 (RpL10 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW249_Pzipt17-loxP-H2BGFP-miniMOS
Plasmid#196063Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.DepositorInsertsH2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR
ExpressionWormAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmMe31B_G
Plasmid#146381PurposeBacterial Expression of DmMe31BDepositorInsertDmMe31B (me31B Fly)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-MBP-DmBam_13-36_AG
Plasmid#148839PurposeMammalian Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRExpressionInsectAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_2
Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H
Plasmid#146503PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
ExpressionInsectAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL472
Plasmid#69882PurposedCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassetteDepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_1-640_AH
Plasmid#148919PurposeInsect Expression of DmGIGYF_1-640DepositorInsertDmGIGYF_1-640 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_J
Plasmid#146709PurposeInsect Expression of DmNot1DepositorInsertDmNot1 (Not1 Fly)
ExpressionInsectMutationThree non silent mutations N605S, K759M, G1999S a…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
ExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC-H6
Plasmid#183777PurposeExpress PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
TagsTurboIDExpressionInsectPromoterUAS-Gal4Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmBicaudalC_P
Plasmid#147163PurposeInsect Expression of DmBicaudalCDepositorInsertDmBicaudalC (BicC Fly)
ExpressionInsectMutation12 silent and three non silent mutattions I331T, …Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_1-694_B
Plasmid#145951PurposeBacterial Expression of DmBelle_1-694DepositorInsertDmBelle_1-694 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF3-V5His6_D
Plasmid#146098PurposeInsect Expression of DmUPF3DepositorInsertDmUPF3 (Upf3 Fly)
ExpressionInsectMutation5 silent and non silent mutation P305S compared t…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only