We narrowed to 1,334 results for: GPCR

1. pCMV-Sport6-CD81-pHluorin

   Plasmid

   #130903

   Purpose

   Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD81-pHluorin (CD81 Human, aequorea victoria)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   pHluorin inserted between Leu-49 and Gly-50 in ex…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRUPATH Triple Gai2

   Plasmid

   #196049

   Purpose

   Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai2-RLuc8 (GNAI2 Human)
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRUPATH Triple Ga11

   Plasmid

   #196059

   Purpose

   Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha11-RLuc8 (GNA11 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRUPATH Triple Ga15

   Plasmid

   #196060

   Purpose

   Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha15-RLuc8 (GNA15 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Sport6-CD63-pHuji

   Plasmid

   #130902

   Purpose

   Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHuji (CD63 Human, synthetic)
   

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Gln-36 and Leu-37 in extra…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRUPATH Triple GaZ

   Plasmid

   #196053

   Purpose

   Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaz-RLuc8 (GNAZ Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Sport6-CD81-pHuji

   Plasmid

   #130904

   Purpose

   Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD81-pHuji (CD81 Human, Synthetic)
   

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Leu-49 and Gly-50 in extra…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Sport6-CD9-pHuji

   Plasmid

   #130906

   Purpose

   Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD9-pHuji (CD9 Human, Synthetic)
   

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Asn50 and Asn51 in extrace…

   Promoter

   CMV

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1/opto-b2AR-EYFP

   Plasmid

   #20948

   Purpose

   Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

   Depositor

   Karl Deisseroth

   Article

   Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

   Insert

   opto-b2AR
   

   Expression

   Mammalian

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-a1AR
    

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-OR51E1

    Plasmid

    #22323

    Depositor

    Hiroaki Matsunami

    Article

    Saito et al Sci Signal. 2009 . 2(60):ra9.

    Insert

    OR51E1 (OR51E1 Human)
    

    Tags

    Rho

    Expression

    Mammalian

    Available Since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2-OXER1-mKate2

    Plasmid

    #226713

    Purpose

    overexpression red fluorescent OXER1 protein in zebrafish larvae

    Depositor

    Philipp Niethammer

    Article

    Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

    Insert

    OXER1 (OXER1 Human)
    

    Use

    Unspecified; Zebrafish expression

    Tags

    mKate2

    Promoter

    SP6

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1D4-hrGFP II

    Plasmid

    #71317

    Purpose

    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.

    Depositor

    Belinda Chang

    Article

    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1

    Type

    Empty backbone

    Tags

    1D4 epitope (TETSQVAPA) and IRES-hrGFPII

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-hsyn-NE2m

    Plasmid

    #208686

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hsyn-NE2h

    Plasmid

    #208687

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

    Depositor

    Yulong Li

    Has Service

    AAV5

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pminiTol2-HuC-NE2h

    Plasmid

    #208689

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Cloning vector

    Promoter

    Huc

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pminiTol2-HuC-NE2m

    Plasmid

    #208688

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    Cloning vector

    Promoter

    Huc

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDisplay-NE2m-IRES-mCherry-CAAX

    Plasmid

    #208690

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDisplay-NE2h-IRES-mCherry-CAAX

    Plasmid

    #208691

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MRGPRX1-Tango

    Plasmid

    #66439

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRX1 (MRGPRX1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only