We narrowed to 11,191 results for: Vars;

1. pAG416GAL-mRuby3

   Plasmid

   #166141

   Purpose

   mRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-His10-ccdB-SNAP

   Plasmid

   #166146

   Purpose

   Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST17-His10-Smt3-ccdB

   Plasmid

   #166147

   Purpose

   Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST17-His10-Smt3-ccdB-SNAP

   Plasmid

   #166148

   Purpose

   Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Smt3 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST17-His10-Smt3-DDX5(kf)-SNAP

   Plasmid

   #166149

   Purpose

   Expression of N. furzeri DDX5 in E. coli

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5
   

   Use

   Tags

   His10-Smt3 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

   Plasmid

   #166150

   Purpose

   Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5ΔPrD(1-535)
   

   Use

   Tags

   His10-Smt3 and SNAP

   Expression

   Bacterial

   Mutation

   ΔPrD(1-535)

   Promoter

   T7

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

   Plasmid

   #166151

   Purpose

   Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5ΔIDR(1-483)
   

   Use

   Tags

   His10-Smt3 and SNAP

   Expression

   Bacterial

   Mutation

   ΔIDR(1-483)

   Promoter

   T7

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTln1-F2-pET151

   Plasmid

   #177870

   Purpose

   Expresses mouse Talin 1 F2 domain in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2 domain (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTln1-F2F3-pET151

   Plasmid

   #177877

   Purpose

   Expresses mouse Talin 1 F2F3 domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 domain (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSJ982

    Plasmid

    #176622

    Purpose

    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

    Insert

    Ub-MPGLW-Fbp1
    

    Use

    Tags

    Ubiquitin N-terminal fusion and triple flag tag C…

    Expression

    Yeast

    Mutation

    Changed first 4 N-terminal amino acid residues of…

    Promoter

    TDH3tc3

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRD400

    Plasmid

    #163107

    Purpose

    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_eYFP_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRD403

    Plasmid

    #163108

    Purpose

    Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    mScarlet-I_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRD128

    Plasmid

    #163097

    Purpose

    Expression of mTurquoise2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    mTurquoise2_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRD129

    Plasmid

    #163098

    Purpose

    Expression of mEos3.2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    mEos3.2_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRD188

    Plasmid

    #163099

    Purpose

    Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEos3.2_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRD198

    Plasmid

    #163101

    Purpose

    Expression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    mEGFP_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRD396

    Plasmid

    #163103

    Purpose

    Expression of eYFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    eYFP_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRD438

    Plasmid

    #168464

    Purpose

    For the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mEos3.2-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MEN2A-MAC

    Plasmid

    #139634

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    MEN2A (RET Human)
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Mammalian

    Mutation

    C634W

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP C1 SADBs shRNA Resistant

    Plasmid

    #67155

    Purpose

    pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    nucleotide 294 C-T, nucleotide 297 C-G

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only