We narrowed to 922 results for: cas12a

1. pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

   Plasmid

   #140226

   Purpose

   Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   Luciferase + crIFNg
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-P25

   Plasmid

   #120036

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   YFP-LID
   

   Use

   Pcr template

   Tags

   YFP-LID

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-Z11

   Plasmid

   #120054

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet-i
   

   Use

   Pcr template

   Tags

   mScarlet-i

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-U6-crRNA4n96

   Plasmid

   #123640

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   crRNA scaffold 4n96
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-P32

   Plasmid

   #120043

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   S-Tag
   

   Use

   Pcr template

   Tags

   S-Tag

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUPD2 AtUBQ10 (GB2478)

   Plasmid

   #160594

   Purpose

   UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtUBQ10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMaCTag-Z25

   Plasmid

   #120068

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   YFP-LID
   

   Use

   Pcr template

   Tags

   YFP-LID

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMaCTag-P19

   Plasmid

   #124789

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry-mNeonGreen
   

   Use

   Pcr template

   Tags

   mCherry-mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-U6-crRNA1

   Plasmid

   #123639

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   crRNA scaffold 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-P30

    Plasmid

    #120041

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    VSV-G
    

    Use

    Pcr template

    Tags

    VSV-G

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Px-PhiYFP-triplex-28-M13-28-pA

    Plasmid

    #202047

    Purpose

    Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, Csy4 28nt sites, and cloning site for gRNA to activate downstream targets.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    PhiYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a-Triplex-28-M13-28-pA

    Plasmid

    #202041

    Purpose

    Encodes a non-coding transcript expressed from the EF1a promoter that has a 3' UTR containing a MALAT1 triplex, Csy4 binding sites ("28"), and a cloning site for a Cas9 gRNA.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    Triplex-28-M13-28 (noncoding)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Px-PhiYFP-triplex-DR-M13-DR-pA

    Plasmid

    #202046

    Purpose

    Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, dCas12a DRs, and cloning site for crRNA to activate downstream targets.

    Depositor

    Darren Cusanovich

    Article

    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

    Insert

    PhiYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaCTag-Z19

    Plasmid

    #124791

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-Z32

    Plasmid

    #120075

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    S-Tag
    

    Use

    Pcr template

    Tags

    S-Tag

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-Z30

    Plasmid

    #120073

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    VSV-G
    

    Use

    Pcr template

    Tags

    VSV-G

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pN256

    Plasmid

    #188472

    Purpose

    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    eGFP-WPRE-DAISY-4T
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D908A

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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