We narrowed to 2,729 results for: fas

1. human p53-Δ(325-355)

   Plasmid

   #24871

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deletion of aa 325-355

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

2. human p53-(310-360)

   Plasmid

   #24872

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains only the dimeric oligomerization domain …

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pGENE:EFF-1::mCherry::V5

   Plasmid

   #133020

   Purpose

   EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

   Insert

   EFF-1::mCherry::V5
   

   Tags

   V5; mCherry

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. human p53-(1-393)-M340Q-L344R

   Plasmid

   #24863

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Methionine 340 mutated to Glutamine Leucine 344 …

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

5. PX544 SpCas9(del1098-1368)

   Plasmid

   #58889

   Purpose

   Truncated form of SpCas9 without C' domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 1098-1368

   Available Since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pre_IV_CS6_4323_v1

   Plasmid

   #126859

   Purpose

   4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS6_4323_v1
   

   Use

   Synthetic Biology

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX543 SpCas9(del175-307)

   Plasmid

   #58888

   Purpose

   Truncated form of SpCas9 without REC2 domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 175-307

   Available Since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJC-20xUAS-TALE1-VP64-P10

   Plasmid

   #104606

   Purpose

   Expresses TALE1 under UAS control

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   TALE1
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET PI-SceI C-His K403A

   Plasmid

   #195612

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

   Insert

   PI-SceI
   

   Expression

   Bacterial

   Mutation

   A1463G, A1464C

   Promoter

   T7

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBluescript-Q22YU3-KO

    Plasmid

    #170313

    Purpose

    Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 knockout
    

    Use

    Tetrahymena somatic gene knockout vector

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBluescript-Q22MS1-KO

    Plasmid

    #170312

    Purpose

    Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1 knockout
    

    Use

    Tetrahymena somatic gene knockout vector

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-Q22MS1-2xStrep

    Plasmid

    #170314

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22MS1
    

    Tags

    Strep-Tag II

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid

    #48141

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. GFP-Ub KO.G76V

    Plasmid

    #11932

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin KO.G76V (RBX1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Conjugation deficient mutant. All 7 lysines of ub…

    Available Since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

    Plasmid

    #89608

    Purpose

    Expression of Luciferase in human cells. 3rd generation lentiviral vector

    Depositor

    Fernando Fierro

    Article

    Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MNDU3

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pre_II_CS3_7653_v2

    Plasmid

    #126855

    Purpose

    7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffold

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    II_CS3_7653_v2
    

    Use

    Synthetic Biology

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBluescript-IC3-FLAG-Tev-ZZ

    Plasmid

    #170311

    Purpose

    Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Outer Dynein Arm Intermediate Chain 3
    

    Tags

    FLAG, ZZ

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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