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Plasmid#17353DepositorInsert4EHP (EIF4E2 Human)
UseTagsFlagExpressionMammalianMutationK121/130/134/222RPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545AK570A
Plasmid#14105DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Gln545 to Ala and Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2_HindIII
Plasmid#65666PurposeFLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII siteDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K570A
Plasmid#14102DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys570 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533A
Plasmid#14100DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545A
Plasmid#14101DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Gln545 to AlaPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AQ545A
Plasmid#14103DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Gln545 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-GTSE1
Plasmid#224719PurposeFor expression of HA-tagged GTSE1 proteinDepositorInsertGTSE1 (GTSE1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEW107
Plasmid#232796PurposeCOMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2abDepositorUseTagsHis6-3xFLAG, TwinstrepExpressionInsectMutationySet1(762-1080)Promoterpolyhedrin promoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEM-601[insert(+1) TA-rich]
Plasmid#221196Purpose601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601DepositorInsert601[insert(+1)TA-rich]
UseUnspecifiedTagsExpressionMutation601 has an additional mutation added 22 bp from d…PromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[R126A/R130A]
Plasmid#221197PurposeScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutationsDepositorInsertScChd1 (aa118-1274)
UseTags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationR126A, R130APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[∆ChEx]
Plasmid#221199PurposeScChd1-aa142-1274 chromatin remodeler with ChEx domain deletedDepositorInsertScChd1 (aa142-1274)
UseTags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468PromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-SHmOCR
Plasmid#206874PurposeEmpty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusionDepositorTypeEmpty backboneUseTagsHSV + 6xHis and SuperHis (14xHis) + monomeric OCR…ExpressionBacterialMutationPromoterT7Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1A
Plasmid#198168PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-Mcl1_deltaTM
Plasmid#187118Purposeinsect cell expression of Mcl1-deltaTMDepositorInsertMCL-1 (BCL2 Human)
UseTagsStrepExpressionInsectMutationdelta328-350PromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only