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MTK1_016

Plasmid

#123679

Purpose

Encodes ConL6 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L6

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_017

Plasmid

#123680

Purpose

Encodes ConL7 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L7

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHES945

Plasmid

#112643

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

CRY2PHR-GAL4DBD

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Sgf73(1-106)/PET32a

Plasmid

#26962

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sgf73(1-106) (SGF73 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pHES936

Plasmid

#89199

Purpose

Expresses DIG1 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

RST1 (DIG1 Budding Yeast)

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pHES938

Plasmid

#89200

Purpose

Expresses MSG5 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

MSG 5 (MSG5 Budding Yeast)

Use

Yeast integration

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pHES939

Plasmid

#89201

Purpose

Expresses GPA1 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GPA1 (GPA1 Budding Yeast)

Use

Yeast integration

Promoter

GAl1

Availability

Academic Institutions and Nonprofits only

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pHES940

Plasmid

#89202

Purpose

Expresses STE4 under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

STE4 (STE4 Budding Yeast)

Use

Yeast integration

Promoter

GAl1

Availability

Academic Institutions and Nonprofits only

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MTK0_002

Plasmid

#123960

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - 2xHS4 - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_038

Plasmid

#123752

Purpose

Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

R-FlincA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE3-NLS

Plasmid

#138218

Purpose

Nuclear-targeted FRET-based cAMP indicator.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

ICUE3-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSiMPlk_N + pSiMPlk_C

Plasmid

#134310

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

kanR_gp41-1 N-intein + kanR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_009

Plasmid

#123672

Purpose

Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-2xHS4

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

Plasmid

#194217

Purpose

Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

UAS-ybTATA-tagBFP; PGK-mCitrine

Use

Lentiviral

Promoter

GAL4 UAS-ybTATA; PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

Plasmid

#186227

Purpose

mRNA synthesis of rapalog-induced cell adhesion molecule

Depositor

Yoshihiro Morishita

Article

Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

Insert

mRNA synthesis of rapalog-induced cell adhesion molecule

Tags

V5

Expression

Mammalian

Mutation

FRB (T2098L)

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Luke-2

Plasmid

#228900

Purpose

Luke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Carbohydrate binding domain cbm49 protein

Use

Acanthamoeba expression vector

Tags

eGFP

Mutation

none

Promoter

Own

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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MTK4_001

Plasmid

#123810

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge