Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 613 results for: ski

Showing: 581 - 600 of 613 results

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pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

Plasmid

#134291

Purpose

Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30, 280 and 443 to Asparag…

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (416-534)

Plasmid

#134267

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 416-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 396-534

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (396-534)

Plasmid

#134266

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 396-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 396-534

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-534)

Plasmid

#134264

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-534

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-396)

Plasmid

#134265

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-396)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-396

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 CaRhAC T2A tDimer

Plasmid

#101722

Purpose

- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

Depositor

Thomas Oertner

Article

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Inserts

CatRhAC
red fluorescent protein

Use

AAV

Mutation

E497K,C569D

Promoter

human Synapsin1 promotor and ribosomal skip seque…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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TRIB3 gRNA (BRDN0001145861)

Plasmid

#77199

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB3 (TRIB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB3 gRNA (BRDN0001147269)

Plasmid

#77201

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB3 (TRIB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB3 gRNA (BRDN0001146247)

Plasmid

#77202

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB3 (TRIB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB1 gRNA (BRDN0001162240)

Plasmid

#76661

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB1 (TRIB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB1 gRNA (BRDN0001162480)

Plasmid

#76662

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB1 (TRIB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB1 gRNA (BRDN0001162575)

Plasmid

#76663

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB1 (TRIB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB1 gRNA (BRDN0001148054)

Plasmid

#76664

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB1 (TRIB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB3 gRNA (BRDN0001146652)

Plasmid

#77200

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB3 (TRIB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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ePB-Puro_TT-hCDX2-P2A-tdTomato-CAAX

Plasmid

#252249

Purpose

A PiggyBac donor vector for Dox-inducible expression of CDX2 and membrane-localized tdTomato.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

CDX2 (CDX2 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Camta2+exon minimal CMV pCDNA5

Plasmid

#212049

Purpose

LR vector for integration of Camta2+exon(21nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Camta2+exon(21nt) (Camta2 Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kansl3-exon minimal CMV pCDNA5

Plasmid

#212052

Purpose

LR vector for integration of Kansl3-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kansl3-exon (Kansl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kansl3+exon minimal CMV pCDNA5

Plasmid

#212053

Purpose

LR vector for integration of Kansl3+exon(78nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kansl3+exon(78nt) (Kansl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kdm1a+exon minimal CMV pCDNA5

Plasmid

#212055

Purpose

LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a+exon(12nt) (Kdm1a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5

Plasmid

#212091

Purpose

LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt) (Chtop Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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