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pCri_L830-antiSH2-OptoMB-V416L

Plasmid

#159936

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Mutation

AsLOV2 V416L

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCri_L889-antiSH2-OptoMB-Triple

Plasmid

#159937

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Mutation

AsLOV2 V416I_G528A_N538E

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP42x-2A(sfGFP)

Plasmid

#126986

Purpose

Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL498

Plasmid

#169154

Purpose

Encodes OptoQ-INVRT4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_L706-antiSH2-OptoMB-irFP

Plasmid

#159938

Purpose

Opto-Monobody mammalian expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Lentiviral

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab18

Plasmid

#31740

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab18 (Rab18 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pMAL724

Plasmid

#169151

Purpose

Encodes OptoQ-AMP5

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Rab6

Plasmid

#31734

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab6 (Rab6 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
GFP-Rab8

Plasmid

#31735

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab8 (Rab8 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Tags

SNAP

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15

Plasmid

#159593

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab10

Plasmid

#31737

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab10 (Rab10 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pMAL669

Plasmid

#169148

Purpose

Encodes OptoQ-AMP2

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL716

Plasmid

#169150

Purpose

Encodes OptoQ-AMP4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1

Plasmid

#60385

Purpose

Expression of yeast alpha tubulin (TUB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA GFP

Plasmid

#32119

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2-Cterminal6xHis

Plasmid

#60386

Purpose

Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Tags

6xHIS

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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