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pICE URA3

Plasmid

#122882

Purpose

URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_40

Plasmid

#109239

Purpose

PTac driving TALEsp2

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

TALEsp2

Expression

Bacterial

Promoter

PTac

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSC301b

Plasmid

#31852

Depositor

Yossef Av-Gay

Publication

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (including ATG start codon)

Expression

Bacterial

Promoter

SOD and none

Available since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-GRK6

Plasmid

#23829

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GRK6 (GRK6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FASTKD5

Plasmid

#23560

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FASTKD5 (FASTKD5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CDKL3

Plasmid

#23628

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDKL3 (CDKL3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-PTK9

Plasmid

#23651

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK9 (TWF1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-ADPGK

Plasmid

#23809

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADPGK (ADPGK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221_mmIrf1 (closed)

Plasmid

#160097

Purpose

Expresses murine Irf1 in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Irf1 (Irf1 Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CaP2AIII_pK18

Plasmid

#153421

Purpose

Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

Depositor

Maki Nakayama

Publication

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha constant region (TRAC*02)-P2A

Expression

Bacterial

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mEos2-EB3-7

Plasmid

#57372

Purpose

Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

EB3 (MAPRE3 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3-HA/Bmal L95E

Plasmid

#47344

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L95E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-CKS2

Plasmid

#23841

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKS2 (CKS2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK11

Plasmid

#23816

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK11 (NEK11 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

Plasmid

#163643

Purpose

Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON17 hgRNA
Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)

Use

Lentiviral

Tags

Luc2-P2A-tdTomato-T2A

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

EFS and pU6

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN4-LSM14A

Plasmid

#240022

Purpose

Expresses human LSM14A fused to 3X Flag in mammalian cells

Depositor

Rui Su

Publication

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

LSM14A

Use

Lentiviral

Tags

3X Flag

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGMV-U

Plasmid

#112797

Purpose

Universal BeYDV-derived vector for cloning of up to four gRNAs

Depositor

Humberto Prieto

Publication

Prieto et al In: Cantu D., Walker M. (eds) The Grape

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAV0749

Plasmid

#133483

Purpose

Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pNapin-eGFP

Plasmid

#127797

Purpose

Napin Promoter driven eGFP expression

Depositor

Scott Poethig

Publication

Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

Type

Empty backbone

Expression

Plant

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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