We narrowed to 19,311 results for: Mut

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pDGE502

Plasmid

#153275

Purpose

M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-Occludin

Plasmid

#136637

Purpose

Expression of Occludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

Occludin (OCLN Chicken)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludin

Plasmid

#136638

Purpose

Expression of HAOccludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludin (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-OccludinCT3

Plasmid

#136639

Purpose

Expression of OccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

OccludinCT3 (OCLN Chicken)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludinCT3

Plasmid

#136640

Purpose

Expression of HAOccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludinCT3 (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-RnBarr2

Plasmid

#137798

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Thy1.1-muSOX D219A

Plasmid

#131704

Purpose

encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-muSOX

Expression

Mammalian

Mutation

Aspartic acid 219 mutated to an alanine

Promoter

CMV

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TaNAM-A1

Plasmid

#124574

Purpose

TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1

Use

Topo gateway cloning vector

Promoter

None

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT3

Plasmid

#118985

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

K2-mutSP-K1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428gp160-754*

Plasmid

#123252

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK513

Plasmid

#121470

Purpose

CEN LEU2 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK511

Plasmid

#121468

Purpose

2µm HIS3 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK510

Plasmid

#121465

Purpose

2µm HIS3 CNB1mutEF3

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF3 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 103 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK509

Plasmid

#121464

Purpose

2µm HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK508

Plasmid

#121463

Purpose

2µm HIS3 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK516

Plasmid

#121473

Purpose

CEN LEU2 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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