We narrowed to 9,265 results for: yeast expression

1. p4A8_M59I_T94M_BC

   Plasmid

   #217833

   Purpose

   4A8 variant in barcoded, yeast surface display plasmid

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   4A8 variant Fab
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   VH: M59I, VL: T94M

   Promoter

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOBD2

   Plasmid

   #196826

   Purpose

   Yeast two-hybrid ; Gal4 DNA binding domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Use

   Tags

   Gal4 DNA binding domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMGF130

   Plasmid

   #96971

   Purpose

   S. pombe lem2 knockout cassette with hygro resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   lem2 (lem2 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   whole ORF deletion and replaced with hygro resist…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMGF172

   Plasmid

   #96998

   Purpose

   ish1 mCherry insertion cassette with ura4+ gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + ura4(…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAD-hRubicon

   Plasmid

   #64144

   Purpose

   For yeast two hybrid

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   GAL4 activation domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pHS3

   Plasmid

   #81038

   Purpose

   HO endonuclease expression

   Depositor

   John McCusker

   Insert

   NATMX - HO endonuclease
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pBHM2651

   Plasmid

   #229993

   Purpose

   Carries AID* tag, empty sgRNA, and NEOR marker

   Depositor

   Hiten Madhani

   Insert

   AID* - empty sgRNA - NEOR
   

   Use

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIAeBlue

   Plasmid

   #185891

   Purpose

   In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIEforRed

   Plasmid

   #185892

   Purpose

   In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKS1604

    Plasmid

    #239528

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS1606

    Plasmid

    #239529

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVL1173

    Plasmid

    #239536

    Purpose

    Express CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker

    Depositor

    John Dueber

    Insert

    mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS1620

    Plasmid

    #239551

    Purpose

    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrCPR-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKS1617

    Plasmid

    #239548

    Purpose

    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKS1618

    Plasmid

    #239549

    Purpose

    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVL1172

    Plasmid

    #239535

    Purpose

    Express CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker

    Depositor

    John Dueber

    Insert

    CpPT1(52-408)-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS1619

    Plasmid

    #239550

    Purpose

    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrCPR-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKS1601

    Plasmid

    #239526

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKS1531

    Plasmid

    #239521

    Purpose

    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    tVoGES-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKS1575

    Plasmid

    #239523

    Purpose

    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2

    Depositor

    John Dueber

    Insert

    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge