-
-
-
Nsp3 -mCherry
Plasmid#165131Purposemammalian expression and localizationDepositorInsertNsp3 (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HAP40 1-371
Plasmid#124060PurposeBaculovirus expression vector for HAP40 protein (aa 1-371) in insect cellsDepositorInsertHAP40 (F8A1 Human)
UseBaculovirus expressionTags6x His and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
UseTagsNuclear Localization SignalExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nsp14-EGFP
Plasmid#165119Purposemammalian expression and localizationDepositorInsertNsp14 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp13_GC3opt
Plasmid#157714Purposemammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCas-Cas12m
Plasmid#192274PurposeEncodes MmCas12m under a constitutive promoterDepositorInsertMmCas12
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23108Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nsp10-mCherry
Plasmid#165135Purposemammalian expression and localizationDepositorInsertNsp10 (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
UseTagsHA tag and Histidine tagExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c020
Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorInsertSYK (SYK Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SopB
Plasmid#183657PurposeN-terminally tagged Salmonella Typhimurium SopB for mammalian expressionDepositorInsertsopB (sopB Salmonella enterica serovar Typhimurium)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9
Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b
Plasmid#89898PurposeBacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationPromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-HA-Ub-T2A-Tet3G
Plasmid#202436PurposeBicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)DepositorInsertUbiquitin
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-AlkBH5(66-292)
Plasmid#125732PurposePlasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.DepositorInsertAlkBH5(66-292) (ALKBH5 Human)
UseTags6xHis and EINExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM185A
Plasmid#218126PurposeHybrid circuit displaying hsCRBN bait and driving expression of luxABDepositorInsertsUseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
UseTags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianMutationPromoterCMV,&RhamnoseAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp15
Plasmid#165120Purposemammalian expression and localizationDepositorInsertNsp15 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp4-mCherry
Plasmid#165132Purposemammalian expression and localizationDepositorInsertNsp4 (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLX_307 FDX1
Plasmid#184495PurposeLentivirus expression for FDX1DepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nsp6-mCherry
Plasmid#165133Purposemammalian expression and localizationDepositorInsertNsp6 (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-sfGFP
Plasmid#107057PurposeExpression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationK785RPromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nsp7-mCherry
Plasmid#165134Purposemammalian expression and localizationDepositorInsertNsp7 (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pPB-cT3G-cERP2-NR1H2
Plasmid#192919PurposeBarcoded piggybac transposon vector with Dox-inducible expression of NR1H2DepositorInsertNR1H2 (NR1H2 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-WT1
Plasmid#192906PurposeBarcoded piggybac transposon vector with Dox-inducible expression of WT1DepositorInsertWT1 (WT1 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only
