We narrowed to 7,201 results for: mme

1. pAF146

   Plasmid

   #222377

   Purpose

   Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

   Depositor

   Andrew S. Flies

   Article

   Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

   Insert

   PDL1
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-brwd1

   Plasmid

   #192742

   Purpose

   Gateway entry vector encoding zebrafish brwd1

   Depositor

   Summer Thyme

   Insert

   brwd1 (brwd1 Zebrafish)
   

   Use

   Gateway entry vector

   Mutation

   K144W; D145E; I760T (rs512641576); Y770F (rs50744…

   Promoter

   None

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWSK29-wzzBST

   Plasmid

   #172968

   Purpose

   pWSK29 with Salmonella typhimurium wzzBST gene

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Insert

   wzzB gene from Salmonella enterica serovar Typhimurium
   

   Expression

   Bacterial

   Mutation

   K198E- please see depositor comments

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWSK29-fepEST

   Plasmid

   #172969

   Purpose

   pWSK29 with Salmonella typhimurium fepEST gene

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Insert

   fepE gene from Salmonella enterica serovar Typhimurium
   

   Expression

   Bacterial

   Mutation

   G160S- please see depositor comments

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C413-E26: attB4-SV40p>-attB1r

   Plasmid

   #162923

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using the SV40 promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   SV40 immediate early promoter
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM-Cas9HF1-D1135E

   Plasmid

   #154924

   Purpose

   aTc inducible Cas9HF1 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9HF1-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEE22

   Plasmid

   #161787

   Purpose

   Hi-SC2 reporter delivery plasmid

   Depositor

   François-Xavier Barre

   Article

   Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

   Insert

   Hi-SC2 reporter
   

   Use

   Conjugative plasmid to deliver hi-sc2 reporter

   Mutation

   Himar site included MmeI site, tandem loxP sites,…

   Promoter

   n/a

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pISH-Olfr1395

   Plasmid

   #136731

   Purpose

   Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395

   Depositor

   Peter Mombaerts

   Article

   Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

   Insert

   Olfr1395
   

   Use

   In situ probe

   Mutation

   ISH Probe insert, D175G and L220F- please dep. co…

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo-lambdaN-UPF1-DEAA

   Plasmid

   #136007

   Purpose

   DEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Tags

   LambdaN

   Expression

   Mammalian

   Mutation

   DE647AA and S743A (Please see depositor comments …

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS415

    Plasmid

    #114272

    Purpose

    SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

    Insert

    Cbr-Unc-119
    

    Use

    CRISPR and Cre/Lox

    Mutation

    *please see depositor comment below

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKSJ375

    Plasmid

    #103064

    Purpose

    Expresses ImmE1, immunity to colicin E1

    Depositor

    Karen Jakes, Alan Finkelstein

    Article

    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

    Insert

    colicin E1 immunity protein (pColE1_26, imm E. coli)
    

    Expression

    Bacterial

    Promoter

    E1 immunity protein promoter from ColE1

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-Fv2E-flt4-ns

    Plasmid

    #67695

    Purpose

    middle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domain

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4
    

    Mutation

    no stop codon; D1302E (see depositor's comme…

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

13. 4QL1

    Plasmid

    #61942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5:APC041-G05:C36415 (WDR5 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. SpDJ-1 pET15b

    Plasmid

    #60689

    Purpose

    Recombinant protein expression of DJ-1

    Depositor

    Mark Wilson

    Article

    Madzelan et al FEBS J. 2012 Nov;279(22):4111-20. doi: 1

    Insert

    DJ-1 (SPAC22E12.03c Fission Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

15. 4MI0

    Plasmid

    #60097

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EZH2:JMC033-A03:C216172 (EZH2 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. FLAG-Sec31a W985A #790

    Plasmid

    #42122

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    G742R and W985A NOTE: the mutation is actually W…

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

17. XE5 Xwnt-5A Hind RNA

    Plasmid

    #16872

    Depositor

    Randall Moon

    Article

    Moon et al Development. 1993 Sep. 119(1):97-111.

    Insert

    Xwnt-5A Hind RNA (wnt5a.S Frog)
    

    Use

    Xenopus expression

    Mutation

    Starts with AA 14 (see comments)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pMT-Pygopus

    Plasmid

    #31900

    Depositor

    Robert Tjian

    Article

    Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

    Insert

    Pygopus (pygo Fly)
    

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pMT FRB-Draper INT-GFP

    Plasmid

    #126665

    Purpose

    Inducible expression of a synthetic FRB-Draper receptor in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    FRB-Draper INT domain
    

    Expression

    Insect

    Mutation

    K986N- see depositor comments below

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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