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Addgene

We narrowed to 7,105 results for: mme

Showing: 6041 - 6060 of 7105 results
  1. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. Salsa6f

    Plasmid
    #140188
    Purpose
    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)
    Depositor
    Insert
    Salsa6f
    Use
    Tags
    tdTomato-v5-GCaMP6f
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Immediate Early
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. eGFP-hCOL1A1

    Plasmid
    #140110
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag, K20…
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Insert
    pstS (A17C,I76G,A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Insert
    pstS (A17C,A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6041 - 6060 of 7105 results