162,717 results

1. pMT-IMD-V5-His

   Plasmid

   #63748

   Purpose

   Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   immune deficiency (imd Fly)
   

   Use

   Tags

   V5-6XHis

   Expression

   Insect

   Mutation

   *see comment below

   Promoter

   pMT

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. hNav1.8_pcDNA3.1

   Plasmid

   #182847

   Purpose

   Expresses hNav1.8 in mammalian cells

   Depositor

   Paul DeCaen

   Article

   McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

   Insert

   SCN10A (SCN10A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUb PspCas13b + PspCas13b guide RNA

   Plasmid

   #176305

   Purpose

   Expresses PspCas13b and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   PspCas13b
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   E113K, N265S- please depositor comments

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB18cmBFPAzurite

   Plasmid

   #14034

   Depositor

   Patrick Daugherty

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Insert

   Azurite
   

   Use

   Yeast/bacteria optimized codons

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-3xHA-Puro

   Plasmid

   #207729

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. GFP-p53

   Plasmid

   #12091

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   72R variant of p53

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-puro

   Plasmid

   #17923

   Purpose

   Insect expression vector

   Depositor

   David Sabatini

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Drosophila metallothionein gene promoter drives e…

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

   Plasmid

   #67976

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJB308

   Plasmid

   #107699

   Purpose

   AtU6 Promoter-LwaCas13a-gRNA Scaffold

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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