We narrowed to 74,949 results for: Rest

1. tet-pLKO.puro_shGFP

   Plasmid

   #110421

   Purpose

   shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-DIO-axon-jGCaMP8f

   Plasmid

   #220531

   Purpose

   For Cre-dependent enriched expression of GCaMP8f in axons

   Depositor

   Lin Tian

   Insert

   Axon-DIO-GCaMP8f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJA54 HLA-A*03:01

   Plasmid

   #180451

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*03:01
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRL-TK CXCR4 4x

   Plasmid

   #11307

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

   Insert

   4 bulged bind sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE.is4-mOpn

   Plasmid

   #74410

   Purpose

   Expresses mouse Osteopontin from transcript tv4 in mammalian cells

   Depositor

   Georgios Stathopoulos

   Insert

   mouse Opn (Spp1 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AbVec2.0-mIghg3

   Plasmid

   #127160

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

   Depositor

   Hedda Wardemann

   Insert

   Ighg3 (Ighg3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 MBP prescission LIC cloning vector (HMPKS)

   Plasmid

   #29721

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and Prescission cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe bleo human PPAR gamma 2

   Plasmid

   #11439

   Depositor

   Ronald Kahn

   Insert

   PPAR gamma 2 (PPARG Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCA

   Plasmid

   #17363

   Depositor

   Stephen Goff

   Article

   Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

   Insert

   MMLV proviral DNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

    Plasmid

    #183252

    Purpose

    Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    anti-CD19 scFvFc (FMC-63)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p75-RFP

    Plasmid

    #24092

    Depositor

    Moses Chao

    Insert

    p75NTR (NGFR Human)
    

    Use

    Tags

    DSRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330 Mouse 3' Hp1a gRNA

    Plasmid

    #127898

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-centrin-1

    Plasmid

    #72641

    Purpose

    The plasmid encodes centrin-1 with an N-terminal EGFP tag

    Depositor

    Michel Bornens

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T-1-Optimized UBE1L

    Plasmid

    #165099

    Purpose

    ecoli optimized expression of GST-fusion of human Ube1L

    Depositor

    Arthur Haas

    Insert

    UbeE1L (UBA7 Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCIG3 Rab5CA

    Plasmid

    #78268

    Purpose

    Rab5 Constitutively active (Q79L) expression

    Depositor

    Felicia Goodrum

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDUAL SRBI (GFP)

    Plasmid

    #86979

    Purpose

    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    SCARB1 (SCARB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. XLone-Puro eGFP

    Plasmid

    #140027

    Purpose

    Tunable and temporal expression control of nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX-2T-Rac1-wt

    Plasmid

    #12977

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pJFRC-MUH

    Plasmid

    #26213

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti6MFV SLC40A1

    Plasmid

    #194132

    Purpose

    Expresses MTP1 (SLC40A1) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC40A1 (SLC40A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTCF236 HLA-B*40:01

    Plasmid

    #180463

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*40:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

    Plasmid

    #183249

    Purpose

    Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    Anti-Her2 scFvFc (FRP5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTCF7 HLA-B*07:02

    Plasmid

    #180446

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*07:02
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLIX403-ccdB-G418

    Plasmid

    #158561

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT2/SV-Venus-SIINFEKL

    Plasmid

    #111624

    Purpose

    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

    Depositor

    Jon Yewdell

    Insert

    Venus-LEQLE-SIINFEKL-TEW
    

    Use

    Tags

    LEQLE-SIINFEKL-TEW

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCEV-G2-Km ymNeongreen

    Plasmid

    #193959

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge