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pHAGE-EGFR-G719S

Plasmid

#116256

Purpose

Lentiviral expression of EGFR G719S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

G719S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Kv1.1 K+ channel scFv [K20/78]

Plasmid

#190486

Purpose

Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_HSPA5_WT

Plasmid

#82132

Purpose

Gateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSPA5 (HSPA5 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR2c-V5

Plasmid

#236013

Purpose

for PiggyBac mediated integration and stable expression of hFGFR2c protein

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR2c (FGFR2 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.R470S

Plasmid

#82781

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

R470S; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BCL-XL-pcw107-V5

Plasmid

#64631

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

BCL2L1 (transcript variant 1) (BCL2L1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221_CLN3

Plasmid

#131868

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

CLN3 (CLN3 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-ERBB4

Plasmid

#116736

Purpose

Lentiviral expression of ERBB4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_WT

Plasmid

#81920

Purpose

Gateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway Cloning

Mutation

I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNF_WT

Plasmid

#81824

Purpose

Gateway Donor vector containing TNF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNF (TNF Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CTLA4-COMP5AP-AviTag-9xHis

Plasmid

#157074

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CTLA4 (CTLA4 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-719 CTLA4-GFP R70W

Plasmid

#186124

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

R70W

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_p.P124S

Plasmid

#81703

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway Cloning

Mutation

P124S; I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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SFFV-GATA2-Brd 9

Plasmid

#218993

Purpose

Transcription factor GATA2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

GATA2 (GATA2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(G12V)-IRES-SNAP-p53DD

Plasmid

#233200

Purpose

Retroviral expression of HRAS(G12V) and SNAP-p53DD

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-SNAP-p53DD (Trp53, HRAS Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-SNAP-p53DD-IRES-HRAS(G12V)

Plasmid

#233198

Purpose

Retroviral expression of SNAP-p53DD and HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

SNAP-p53DD-IRES-HRAS(G12V) (HRAS, Trp53 Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EDA2R-COMP5AP-AviTag-9xHis

Plasmid

#157096

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EDA2R (EDA2R Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Worm ABeta-attR5

Plasmid

#160437

Purpose

Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Nematode, Human)

Tags

Cleavage signal

Expression

Bacterial

Mutation

Codon-optimized for expression in C. elegans

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_WT

Plasmid

#81782

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-CLN3_STOP

Plasmid

#161034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

CLN3 (CLN3 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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