We narrowed to 6,523 results for: sas

1. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

   Plasmid

   #167832

   Purpose

   Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   HRAS(N17) (HRAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S17N

   Promoter

   Ubc, TRE

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-D1-IRES-SS-D2-IRES-HA-D3

   Plasmid

   #215119

   Purpose

   Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Inserts

   CCND1 (CCND1 Human)
   CCND2 (CCND2 Human)
   CCND3 (CCND3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-tag, HA-tag, and SS (STREP-STREP)

   Expression

   Mammalian

   Promoter

   IRES and TREG3

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

   Plasmid

   #198465

   Purpose

   Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-XBB.1.5 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

   Plasmid

   #176141

   Purpose

   EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   XRCC1-T2A-PolB (XRCC1, POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP and MYC

   Expression

   Mammalian

   Mutation

   Myc-tagged PolB with mutation in Asp256 to Ala, a…

   Promoter

   EF1A

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

   Plasmid

   #176140

   Purpose

   EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   XRCC1-T2A-PolB (XRCC1, POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP and MYC

   Expression

   Mammalian

   Mutation

   Myc-tagged PolB with mutations Lys35 to Ala, Lys6…

   Promoter

   EF1A

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP12039 - pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2039)

   Plasmid

   #208401

   Purpose

   AiE0354h is an enhancer sequence designed to drive AAV-mediated transgene expression in Vip+ cortical interneurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Lee et al Science. 2023 Oct 13;382(6667):eadf6484.

   Insert

   SYFP2
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   eHGT_354h (enhancer)

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_18GZG

   Plasmid

   #117228

   Purpose

   shble flanked by an endogenous GAPDH promoter and terminator system

   Depositor

   Jackie Collier

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   shble
   

   Use

   Thraustochytrid expression

   Promoter

   GAPDH

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DinoIII-neo

   Plasmid

   #155026

   Purpose

   Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   amino 3'-glycosyl phosphotransferase
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. DinoIII-pat

   Plasmid

   #155028

   Purpose

   Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   Phosphinothricin N-acetyltransferase
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. DinoIII-bsr

    Plasmid

    #155027

    Purpose

    Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

    Depositor

    Senjie Lin

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Insert

    Blasticidin-S deaminase
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC:FCP:ShBle:FCP:EGFP

    Plasmid

    #85987

    Purpose

    Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Inserts

    ShBle
    EGFP
    

    Promoter

    FCP

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLU_103

    Plasmid

    #225785

    Purpose

    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.

    Depositor

    Nili Ostrov

    Insert

    Ptn5_[Tn5]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLU_85

    Plasmid

    #225800

    Purpose

    This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.

    Depositor

    Nili Ostrov

    Insert

    PmarC9_[Himar]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-CAG-rtTA3G-IH

    Plasmid

    #214118

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    trTA3G
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-CAG-rtTAM2-IB

    Plasmid

    #214117

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    rtTAM2
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-NEO-GFP-RFP

    Plasmid

    #157952

    Purpose

    seamless gene editing and component of Piggybac transposase system

    Depositor

    Yan-zhen Bi

    Type

    Empty backbone

    Use

    Mouse Targeting; Gene targeting

    Expression

    Mammalian

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-hVASA-KO-WPRE

    Plasmid

    #214124

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    hKO
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-Brainbow4.2-PGK-HygTK

    Plasmid

    #214126

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    Brainbow
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-hTHp-Cre-pNeo

    Plasmid

    #214122

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    Cre
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cjOCT4-mNG-WPRE

    Plasmid

    #214123

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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