161,255 results

1. pCDH-CMV-mCherry-T2A-Puro

   Plasmid

   #72264

   Purpose

   Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

   Depositor

   Kazuhiro Oka

   Insert

   mCherry-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS1089: mini-AAV.sgRNA.Nme2Cas9

   Plasmid

   #159536

   Purpose

   Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 with single guide RNA cassette
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSXneo 270(T2AG3)

   Plasmid

   #12403

   Depositor

   Titia de Lange

   Article

   Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

   Insert

   270(T2AG3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFPN1-ATG9A

   Plasmid

   #198529

   Purpose

   Expression of ATG9A-GFP fusion protein in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

   Insert

   ATG9A (ATG9A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations in codons 4 and 775

   Promoter

   CMV

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-7 asyn A53T

   Plasmid

   #105727

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A53T (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A53T

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5/FRT/DEST-3xHA/mGli3/Flag

   Plasmid

   #51246

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

8. EGFP-Rubicon

   Plasmid

   #28022

   Depositor

   Qing Zhong

   Article

   Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   3xFlag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX403-ccdB-G418

   Plasmid

   #158561

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid

    #199781

    Purpose

    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

    Depositor

    Sascha Martens

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    GST-Thrombin-TEV-EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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