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pDONR223-ZNF800

Plasmid

#88573

Purpose

Donor Vector containing ZNF800 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF800 (ZNF800 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTBL472

Plasmid

#126457

Purpose

Expresses Cas9-10xFlag-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

Cas9
10x Flag
TdT (DNTT Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgMARK4

Plasmid

#138691

Purpose

Expresses a human MARK4-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 human (MARK4 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF219

Plasmid

#88118

Purpose

Donor Vector containing ZNF219 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF219 (ZNF219 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF830

Plasmid

#88342

Purpose

Donor Vector containing ZNF830 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF830 (ZNF830 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF808

Plasmid

#101470

Purpose

Donor Vector containing ZNF808 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF808 (ZNF808 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF331

Plasmid

#88442

Purpose

Donor Vector containing ZNF331 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF331 (ZNF331 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF785

Plasmid

#88459

Purpose

Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF785 (ZNF785 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF232

Plasmid

#88305

Purpose

Donor Vector containing ZNF232 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF232 (ZNF232 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_OTUD6A_WT

Plasmid

#81813

Purpose

Gateway Donor vector containing OTUD6A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OTUD6A (OTUD6A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLCHKO_TK1_HPRT1_Lb_4

Plasmid

#155062

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF442

Plasmid

#101455

Purpose

Donor Vector containing ZNF442 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF442 (ZNF442 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF366

Plasmid

#88019

Purpose

Donor Vector containing ZNF366 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF366 (ZNF366 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PRKCE_WT

Plasmid

#82227

Purpose

Gateway Donor vector containing PRKCE , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCE (PRKCE Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF175

Plasmid

#88551

Purpose

Donor Vector containing ZNF175 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF175 (ZNF175 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBM11_WT

Plasmid

#81784

Purpose

Gateway Donor vector containing RBM11 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM11 (RBM11 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_p.F88S

Plasmid

#81473

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

F88S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_p.G109V

Plasmid

#81478

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

G109V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC2A3_p.K49N

Plasmid

#81461

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Mutation

K49N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RGR_WT

Plasmid

#81845

Purpose

Gateway Donor vector containing RGR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RGR (RGR Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RAB42_WT

Plasmid

#81796

Purpose

Gateway Donor vector containing RAB42 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAB42 (RAB42 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SGK1_p.A48G

Plasmid

#81430

Purpose

Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SGK1 (SGK1 Human)

Use

Gateway entry vector

Mutation

A48G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SGK1_p.F27L

Plasmid

#81391

Purpose

Gateway Donor vector containing SGK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SGK1 (SGK1 Human)

Use

Gateway entry vector

Mutation

F27L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EphA2 1-427 pcDNA3.1

Plasmid

#102737

Purpose

Expression of N terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (1-427aa) (Epha2 Mouse)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C13orf37_WT

Plasmid

#81872

Purpose

Gateway Donor vector containing MZT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C13orf37 (MZT1 Human)

Use

Gateway entry vector

Mutation

S5G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4-CMV-myc-XPO1

Plasmid

#242469

Purpose

Expresses human XPO1 protein with an N-terminal cMyc tag.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

XPO1 (XPO1 Human)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-FLAG-XPO1

Plasmid

#242459

Purpose

Expresses human XPO1 with an N-terminal FLAG tag for pull-down assays.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

XPO1 (XPO1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-YB_TATA)-Halo-p53-BGH_pKG2745

Plasmid

#246339

Purpose

203-bp DIAL Reporter Plasmid with YB_TATA expressing Halo-p53 in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

Halo-p53 (Trp53 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Mutation

WT

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG2744

Plasmid

#246340

Purpose

203-bp DIAL Reporter Plasmid with YB_TATA expressing mCherry-GS-HRasG12V in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Synthetic Biology

Expression

Mammalian

Mutation

G12V

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-eGFP

Plasmid

#227237

Purpose

Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

eGFP (eGFP )

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge