We narrowed to 10,402 results for: plasmids 101

1. pRiboI-APEX2m

   Plasmid

   #176843

   Purpose

   Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Use

   Integrating

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HCMVgL-FLAG (Merlin)

   Plasmid

   #136450

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein L (UL115 Human betaherpesvirus 5)
   

   Tags

   Short SGGSG linker and FLAG tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEW4: pET-32a Dot1L(2-386)

   Plasmid

   #124099

   Purpose

   Dot1L(2-386) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-386) (DOT1L Human)
   

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

   Plasmid

   #67295

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

   Plasmid

   #184633

   Purpose

   Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Aldh1a1 (Aldh1a1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRibo-APEX2m

   Plasmid

   #176842

   Purpose

   Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAGGS-Flex-GFP

   Plasmid

   #197883

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

   Plasmid

   #67292

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Myt1L (Myt1l Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE

   Plasmid

   #67293

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Myt1L (Myt1l Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian and Yeast

   Promoter

   PGK

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBstSK+IRES-V5-IPpoI (mutant)

    Plasmid

    #206322

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag-mutated IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein (mutant version)

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

    Plasmid

    #158989

    Purpose

    Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-SID4X
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AID-BFP-loxP_myo2_neoR

    Plasmid

    #194054

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Tags

    bfp

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLKO.1-UPF3B-CDS

    Plasmid

    #136045

    Purpose

    UPF3B shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GAAGCCTTGTTCCGATCTAAT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3B (UPF3B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMX-eSOX17WHC

    Plasmid

    #118839

    Purpose

    Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX17WHC (Sox17 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    WHC: L46W, V53H, E57C

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSR06

    Plasmid

    #69152

    Purpose

    read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0 and codon-optimzed index…

    Promoter

    rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLP110_AAV Scramble Control

    Plasmid

    #239419

    Purpose

    Negative control AAV vector for SaCas9-based CRISPR KO

    Depositor

    Subhojit Roy

    Article

    Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

    Insert

    Scramble control
    

    Use

    AAV

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFNC-2

    Plasmid

    #227667

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. KmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    KmR
    

    Expression

    Bacterial

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

    Plasmid

    #229793

    Purpose

    Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFNC-4

    Plasmid

    #227668

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    SmR
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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