We narrowed to 17,133 results for: Por

1. pTn7CdrA-gfp(ASV)C

   Plasmid

   #111617

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2-DelGFP

   Plasmid

   #133302

   Purpose

   EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-RhoA FLARE.sc Biosensor WT

   Plasmid

   #12150

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

   Plasmid

   #205565

   Purpose

   Express human NEDD4L (delta C2 isoform)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   delta C2 isoform

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti-CMV-mCherry-T2A-GFP

   Plasmid

   #109427

   Purpose

   A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

   Plasmid

   #124494

   Purpose

   Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Caspase-1 cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-AC EJ7-GFP

   Plasmid

   #113617

   Purpose

   Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   EJ7-GFP variant of EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK-caCas9-NatMX-Neut5L

   Plasmid

   #89576

   Purpose

   caCas9 integrating vector into the Neut5L locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dat - EGFP - Tol2

   Plasmid

   #85418

   Purpose

   for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

   Depositor

   Marc Ekker

   Article

   Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

   Insert

   dopamine transporter (slc6a3 Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Promoter

   dat

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pAG654 pFAST

    Plasmid

    #172861

    Purpose

    Expresses pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. XLone-ETV2

    Plasmid

    #135763

    Purpose

    expresses human ETV2 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    ETV2 (ETV2 Human)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    TRE3G

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-NINJ1

    Plasmid

    #201179

    Purpose

    Expression of NINJ1 gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115211

    Purpose

    Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PsmCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-mScarlet-SH3PXD2A

    Plasmid

    #215534

    Purpose

    Expression vector with Tks5 tagged with N-terminal mScarlet

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    SH3PXD2A (SH3PXD2A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLexM-SLC7A11_GFP

    Plasmid

    #197995

    Purpose

    Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

    Depositor

    Simon Newstead

    Article

    Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Tags

    FLAG tag and TEV-GFP-His8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. P445-FBP_17

    Plasmid

    #139708

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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